HSP60 (D85) Antibody

• 产品编号：CST-4869S      品牌：CST       原厂货号：4869S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

HSP60 (D85) Antibody抗体识别内源性的HSP60总蛋白。此抗体与其它HSP蛋白没有交叉反应。该多克隆抗体用与人类HSP60蛋白中Asp85附近氨基酸序列对应的人工合成肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。在原核和真核生物生物合成和细胞应激时分子伴侣可以防止蛋白质的错误折叠和堆积（1-3）。HSP60主要被认为是线粒体蛋白，对关键蛋白质转入线粒体后的正确折叠很重要（4）。目前比较清楚的是HSP60在很多细胞的细胞质中存在，并且可以被应激、炎症和免疫反应诱导，自身抗体与老年痴呆、冠心病、多发性硬化和糖尿病均相关（5-8）。

1. Hartl, F.U. (1996) Nature 381, 571-579.
2. Bukau, B. and Horwich, A.L. (1998) Cell 92, 351-366.
3. Hartl, F.U. and Hayer-Hartl, M. (2002) Science 295, 1852-1858.
4. Jindal, S. et al. (1989) Mol. Cell Biol. 9, 2279-2283.
5. Itoh, H. et al. (2002) Eur. J. Biochem. 269, 5931-5938.
6. Gupta, S. and Knowlton, A.A. J. Cell Mol. Med. 9, 51-58.
7. Deocaris, C.C. et al. (2006) Cell Stress Chaperones 11, 116-128.
8. Lai, H.C. et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292, E292-E297.

原厂资料：

Specificity / Sensitivity

HSP60 (D85) Antibody detects endogenous levels of total HSP60 protein. This antibody does not cross-react with other HSPs.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Asp85 of human HSP60. Antibodies are purified by protein A and peptide affinity chromatography.

Background

In both prokaryotic and eukarytic cells the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones (1-3). HSP60 has primarily been known as a mitochondrial protein that is important for folding key proteins after import into the mitochondria (4). It is now clear that a significant amount of HSP60 is also present in the cytosol of many cells and that it is induced by stress, inflammatory and immune responses, autoantibodies correlated with Alzheimer's, coronary artery diseases, MS, and diabetes (5-8).

1. Hartl, F.U. (1996) Nature 381, 571-579.
2. Bukau, B. and Horwich, A.L. (1998) Cell 92, 351-366.
3. Hartl, F.U. and Hayer-Hartl, M. (2002) Science 295, 1852-1858.
4. Jindal, S. et al. (1989) Mol. Cell Biol. 9, 2279-2283.
5. Itoh, H. et al. (2002) Eur. J. Biochem. 269, 5931-5938.
6. Gupta, S. and Knowlton, A.A. J. Cell Mol. Med. 9, 51-58.
7. Deocaris, C.C. et al. (2006) Cell Stress Chaperones 11, 116-128.
8. Lai, H.C. et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292, E292-E297.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody