E2A Antibody

• 产品编号：CST-4865S      品牌：CST       原厂货号：4865S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

E2A抗体能够检测内源水平E2A蛋白总体水平。此多克隆抗体是通过合成人源对应的E2A Leu475位点周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。E2A是转录因子E-蛋白家族的成员, 是基本的螺旋-环-螺旋(bHLH)蛋白亚类，能够特异性的结合到与E-盒子一致保守的序列上(1,2)。可变剪切产生了两个E2A 亚型(E47 和 E12)，他们活跃的参与到B细胞系决定、B淋巴细胞成熟、IgK V-J重组、外周血B细胞的发展与肿瘤的抑制过程中(3)。E2A在G1到免疫球蛋白多样化分化过程中起到顺式作用(4)。E2A基因染色体的易位导致了多种融合蛋白的表达并与小儿急性淋巴细胞性白血病有关(5)。

1. Murre, C. (2005) Nat Immunol 6, 1079-86.
2. Bain, G. et al. (2001) Nat Immunol 2, 165-71.
3. Schwartz, R. et al. (2006) Proc Natl Acad Sci U S A 103, 9976-81.
4. Yabuki, M. et al. (2009) J Immunol 182, 408-15.
5. LeBrun, D.P. (2003) Front Biosci 8, s206-22.

原厂资料：

Specificity / Sensitivity

E2A Antibody detects endogenous levels of total E2A protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu475 of human E2A protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

E2A is a member of the E-protein family of transcription factors, a subclass of basic helix-loop-helix (bHLH) proteins that bind specifically to E-box consensus sequences (1,2). Alternative splicing generates two E2A isoforms (E47 and E12) that are actively involved in B-cell lineage commitment, B-lymphocyte maturation, IgK V-J rearrangement, peripheral B-cell development, and tumor suppression (3). E2A acts in cis during G1 to promote immunoglobulin gene diversification (4). Chromosomal translocations involving the E2A gene result in the expression of multiple fusion proteins and are associated with many cases of pediatric acute lymphoblastic leukemia (5).

1. Murre, C. (2005) Nat Immunol 6, 1079-86.
2. Bain, G. et al. (2001) Nat Immunol 2, 165-71.
3. Schwartz, R. et al. (2006) Proc Natl Acad Sci U S A 103, 9976-81.
4. Yabuki, M. et al. (2009) J Immunol 182, 408-15.
5. LeBrun, D.P. (2003) Front Biosci 8, s206-22.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.