Phospho-Zyxin (Ser142/143) Antibody

• 产品编号：CST-4863S      品牌：CST       原厂货号：4863S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Zyxin (Ser142/143)抗体仅能识别142位，143位丝氨酸磷酸化或两个氨基酸残基同时同时磷酸化的内源性zyxin蛋白。合成对应人zyxin蛋白142位丝氨酸及其邻近氨基酸残基序列的磷酸多肽免疫动物获得单克隆抗体。抗体由蛋白A和肽亲和层析纯化。zyxin (斑联蛋白)家族包括LIMD1，ajuba，trip6和zyxin ，每个蛋白在羧基末端都有3个LIM结构域。Zyxin 家族成员与肌动蛋白丝细胞骨架相关联，是细胞间连接和整联蛋白介导的粘附复合物的组成部分。它们在细胞核内外穿梭发挥转录激活作用（1）。Zyxin涉及调控肌动蛋白丝机械压力诱导下在黏着斑部位的多聚化（2）并且调控黏附和迁徙，可能是通过向黏着斑募集Ena/VASP蛋白而实现的（3）。Zyxin与肿瘤抑制因子myopodin结合并可能调控器功能，myopodin能够抑制肿瘤生长和转移（4）。Zyxin可以被Akt在第142位丝氨酸磷酸化（5）。

1. Wang, Y. and Gilmore, T.D. (2003) Biochim Biophys Acta 1593, 115-20.
2. Hirata, H. et al. (2008) J Cell Sci 121, 2795-804.
3. Hoffman, L.M. et al. (2006) J Cell Biol 172, 771-82.
4. Yu, Y.P. and Luo, J.H. (2006) Cancer Res 66, 7414-9.

原厂资料：

Specificity / Sensitivity

Phospho-Zyxin (Ser142/143) Antibody recognizes endogenous levels of zyxin protein only when phosphorylated at Ser142, Ser143 or both residues.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser142 of human zyxin protein. Antibodies are purified using protein A and peptide affinity chromatography.

Background

The zyxin family of proteins includes LIMD1, ajuba, trip6, and zyxin, each of which contains three LIM domains at the carboxy terminus. Zyxin family members associate with the actin cytoskeleton and are components of both the cell-cell junction adhesive complex and the integrin-mediated adhesive complex. They shuttle in and out of the nucleus where they may function in transcriptional activation (1). Zyxin is involved in the regulation of mechanical force-induced actin polymerization at focal adhesions (2) and in the regulation of adhesion and migration, possibly through the recruitment of Ena/VASP proteins to focal adhesions (3). Zyxin interacts with and may regulate the function of the tumor suppressor myopodin, which inhibits tumor growth and metastasis (4). Zyxin may be phosphorylated by Akt at Ser142 (5).

1. Wang, Y. and Gilmore, T.D. (2003) Biochim Biophys Acta 1593, 115-20.
2. Hirata, H. et al. (2008) J Cell Sci 121, 2795-804.
3. Hoffman, L.M. et al. (2006) J Cell Biol 172, 771-82.
4. Yu, Y.P. and Luo, J.H. (2006) Cancer Res 66, 7414-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.