DOCK180 (C4C12) Rabbit mAb

• 产品编号：CST-4846S      品牌：CST       原厂货号：4846S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

DOCK180 (C4C12) Rabbit mAb兔单抗检测内源性DOCK180总蛋白。通过人工合成人源DOCK180蛋白羧基末端相应的多肽片段去免疫动物从而制备出单克隆抗体。DOCK180蛋白和它的同伴ELMO1蛋白彼此直接相互作用，对于小GTPase Rac从而形成一个不规则的鸟嘌呤核苷酸交换因子(GEF)的两部分 (1)。Rac的激活发生在p130 Cas和Crk的连接中，这将于DOCK180蛋白形成复合物，其将把粘着斑作为目标 (2,3)。DOCK180蛋白也通过结合磷酸肌醇被招募到质膜 (4)。ELMO1 蛋白可能的功能作为DOCK180的蛋白酶体依赖降解的抑制剂，从而调节actin细胞骨架的重组（5）。通过WAVE家族蛋白、整合素信号、板状伪足和丝状伪足的形成和吞噬作用或细胞迁移等进程的调节，小范围 Rac的激活允许actin成核(6-8)。

1. Brugnera, E. et al. (2002) Nat. Cell Biol. 4, 574-582.
2. Matsuda, M. et al. (1996) J. Biol. Chem. 271, 14468-14472.
3. Côté, J.F. et al. (2005) Nat. Cell Biol. 7, 797-807.
4. Makino, Y. et al. (2006) J. Cell Sci. 119, 923-932.
5. Takenawa, T. and Miki, H. (2001) J. Cell Sci. 114, 1801-1809.
6. Albert, M.L. et al. (2000) Nat. Cell Biol. 2, 899-905.
7. Gustavsson, A. et al. (2004) J. Biol. Chem. 279, 22893-22901.

原厂资料：

Specificity / Sensitivity

DOCK180 (C4C12) Rabbit mAb recognizes endogenous levels of total DOCK180 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal sequence of human DOCK180.

Background

DOCK180 and its partner, ELMO1, interact directly with one another to form an atypical two-part guanine nucleotide exchange factor (GEF) for the small GTPase Rac (1). Rac activation occurs in association with p130 Cas and Crk, which form a complex with DOCK180 that is targeted to focal adhesions (2,3). DOCK180 is also recruited to the plasma membrane by binding to phosphoinositides (4). ELMO1 may function as an inhibitor of proteasome-dependent degradation of DOCK180 at the plasma membrane to regulate reorganization of the actin cytoskeleton (5). Localized Rac activation allows actin nucleation via WAVE family proteins, signaling to integrins, formation of lamellipodia and filopodia, and regulation of processes such as phagocytosis and cell migration (6-8).

1. Brugnera, E. et al. (2002) Nat. Cell Biol. 4, 574-582.
2. Matsuda, M. et al. (1996) J. Biol. Chem. 271, 14468-14472.
3. Côté, J.F. et al. (2005) Nat. Cell Biol. 7, 797-807.
4. Makino, Y. et al. (2006) J. Cell Sci. 119, 923-932.
5. Takenawa, T. and Miki, H. (2001) J. Cell Sci. 114, 1801-1809.
6. Albert, M.L. et al. (2000) Nat. Cell Biol. 2, 899-905.
7. Gustavsson, A. et al. (2004) J. Biol. Chem. 279, 22893-22901.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium
azide. Store at –20°C. Do not aliquot the antibody.