APS Antibody

• 产品编号：CST-4832S      品牌：CST       原厂货号：4832S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

APS兔多抗能检测内源性总APS蛋白水平。这种抗体不会与其他受体/连接蛋白发生交叉反应。该多克隆抗体通过使用与人源APS蛋白胺端周围残基相一致的合成肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。APS是一种包含SH2 、PH结构域的受体蛋白，与Lnk 、SH2-B 蛋白密切相关(1)。APS被认为是许多受体酪氨酸激酶的底物，这些激酶包括TrkA、胰岛素受体、c-Kit 、 PDGF受体(2)。APS的酪氨酸磷酸化为下游信号传导组件提供了连接位点，从而能够调节各种信号传递通路。APS在RTK信号传导过程中扮演着完全不同的角色。APS的过量表达会抑制PDGF诱导的有丝分裂发生，这个现象可能是由于APS/c-Cbl调节的PDGF受体的降解(3)。然而APS在胰岛素的刺激下能促进有丝分裂的发生(4)。APS调节不同的RTKs 信号传导过程中最显著的差别在于与各自受体的反应模式不同。

1. Liu, J. et al. (2002) Mol. Cell. Biol. 22, 3599-3609.
2. Qian, X. and Ginty, D. (2001) Mol. Cell. Biol. 21, 1613-1620.
3. Wollberg, P. et al. (2003) Biochem. J. 370, 1033-1038.
4. Ahmed, Z. and Pillay, T.S. (2001) Biochem. Soc. Trans. 29, 529-534.

原厂资料：

Specificity / Sensitivity

APS Antibody detects endogenous levels of total APS. This antibody does not cross-react with other adaptor/docking proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino-terminus of human APS. Antibodies are purified by protein A and peptide affinity chromatography.

Background

APS is an SH2 and PH domain-containing adaptor protein closely related to Lnk and SH2-B (1). APS was identified as a substrate for many receptor tyrosine kinases including TrkA, insulin receptor, c-Kit and PDGF receptor (2). Tyrosine phosphorylation of APS provides docking sites for downstrean signaling components, mediating diverse signaling pathways. APS plays quite different roles in RTK signaling. Overexpression of APS has been shown to inhibit PDGF-induced mitogenicity, which may result from APS/c-Cbl-mediated PDGF receptor degradation (3). However, APS promotes enhanced mitogenicity in response to insulin stimulation (4). The striking difference in APS-mediated signaling between the different RTKs could lie in the mode of interaction with the respective receptor.

1. Liu, J. et al. (2002) Mol. Cell. Biol. 22, 3599-3609.
2. Qian, X. and Ginty, D. (2001) Mol. Cell. Biol. 21, 1613-1620.
3. Wollberg, P. et al. (2003) Biochem. J. 370, 1033-1038.
4. Ahmed, Z. and Pillay, T.S. (2001) Biochem. Soc. Trans. 29, 529-534.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody