hnRNP LL Antibody

• 产品编号：CST-4783S      品牌：CST       原厂货号：4783S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

hnRNP LL Antibody检测内源性hnRNP LL总蛋白水平。通过人工合成人源hnRNP LL蛋白氨基末端序列相应的多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。Heterogeneous nuclear ribonucleoprotein L-like (hnRNP LL)是一个细胞核RNA结合蛋白，它与hnRNP L蛋白共有69% 氨基酸同源序列，hnRNP L是hnRNP复合物的组分，能够调控mRNA生成，包装以及加工(1)。 hnRNP LL蛋白在活化的T细胞中被诱导，并且可作为对T细胞发育和激活至关重要的酪氨酸磷酸酶CD45选择性剪接的关键调控因子(2)。hnRNP LL 还能够调控CD44 和Stat5a的选择性剪接(2)。hnRNP LL有四个异构体，都是通过选择性剪接产生的，并且在人类组织中广泛表达(3)。

1. Pinol-Roma, S. et al. (1989) J Cell Biol 109, 2575-87.
2. Oberdoerffer, S. et al. (2008) Science 321, 686-91.
3. Shur, I. et al. (2004) Gene 334, 113-21.

原厂资料：

Specificity / Sensitivity

hnRNP LL Antibody detects endogenous levels of total hnRNP LL protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human hnRNP LL protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Heterogeneous nuclear ribonucleoprotein L-like (hnRNP LL) is a nuclear RNA-binding protein that shares 69% amino acid homology with hnRNP L, a component of hnRNP complex that regulates mRNA formation, packaging, and processing (1). hnRNP LL is induced in activated T cells and functions as a critical regulator of alternative splicing of CD45, a tyrosine phosphatase crucial for T cell development and activation (2). hnRNP LL also regulates the splicing of CD44 and Stat5a (2). The four isoforms of hnRNP LL are generated by alternative splicing and are widely expressed in human tissues (3). 

1. Pinol-Roma, S. et al. (1989) J Cell Biol 109, 2575-87.
2. Oberdoerffer, S. et al. (2008) Science 321, 686-91.
3. Shur, I. et al. (2004) Gene 334, 113-21.

注意事项：

Storage: Supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl,
100 µg/ml BSA and 50% glycerol. Store at -20°C.
Do not aliquot the antibody.