Phospho-Filamin A (Ser2152) Antibody

• 产品编号：CST-4761S      品牌：CST       原厂货号：4761S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Filamin A (Ser2152) Antibody仅检测在serine 2152位点磷酸化的内源性Filamin A蛋白。该抗体也与在serine 2146位点磷酸化的filamin C蛋白发生交叉反应。通过人工合成人源filamin A蛋白Ser2152位点和人源filamin C蛋白Ser2146位点周围相应的磷酸化片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。Filamins蛋白是二聚化actin结合蛋白的家族，其功能是作为细胞粘附位点的结构性组件。对于亚细胞内信号分子的靶点它们也作为一个支架(1)。actin结合域(alpha-actinin domain)是定位在特有的氨基末端，接着是多达24串联的大约96残基重复序列，而二聚体化结构域是位于该蛋白羧基末端。除actin filaments之外，filamins与其它结构上分子和信号分子例如beta-integrins、Rho/Rac/Cdc42、PKC和insulin receptor有关联，但主要通过部分羧基末端区域(1-3)。高丰度的Filamin A蛋白和低丰度的filamin B蛋白是广泛表达的亚型，而filamin C 蛋白是在肌肉中主要表达(1)。filamin A蛋白的Serine 2152位点通过PAK1激酶磷酸化，而这个磷酸化对于PAK1介导的actin细胞骨架重组是需要的(4)。

1. Stossel, T.P. et al. (2001) Nat Rev Mol Cell Biol 2, 138-45.
2. Tigges, U. et al. (2003) J Biol Chem 278, 23561-9.
3. He, H.J. et al. (2003) J Biol Chem 278, 27096-104.
4. Vadlamudi, R.K. et al. (2002) Nat Cell Biol 4, 681-90.

原厂资料：

Specificity / Sensitivity

Phospho-Filamin A (Ser2152) Antibody detects endogenous levels of filamin A only when phosphorylated at serine 2152. This antibody also reacts with filamin C when it is phosphorylated at serine 2146.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser2152 of human filamin A and Ser2146 of human filamin C. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Filamins are a family of dimeric actin binding proteins that function as structural components of cell adhesion sites. They also serve as a scaffold for subcellular targeting of signaling molecules (1). The actin binding domain (alpha-actinin domain) is located at the very amino-terminus, followed by as many as 24 tandem repeats of about 96 residues, and the dimerization domain is located at the carboxy-terminal end of th protein. In addition to actin filaments, filamins associate with other structural and signaling molecules such as beta-integrins, Rho/Rac/Cdc42, PKC, and insulin receptor, primarily through their carboxy-terminal half (1-3). Filamin A, the most abundant, and filamin B, less abundant, are widely expressed isoforms, while filamin C is predominantly expressed in muscle (1). Serine 2152 of filamin A is phosphorylated by PAK1, and this phosphorylation is required for PAK1-mediated actin cytoskeleton reorganization (4).

1. Stossel, T.P. et al. (2001) Nat Rev Mol Cell Biol 2, 138-45.
2. Tigges, U. et al. (2003) J Biol Chem 278, 23561-9.
3. He, H.J. et al. (2003) J Biol Chem 278, 27096-104.
4. Vadlamudi, R.K. et al. (2002) Nat Cell Biol 4, 681-90.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody