Nestin (Rat-401) Mouse mAb

• 产品编号：CST-4760S      品牌：CST       原厂货号：4760S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Nestin (Rat-401) Mouse mAb识别内源性的总nestin蛋白。该单克隆抗体由大鼠脊髓E15免疫动物而制成。Nestin是中间丝纤维家族成员，与神经纤维蛋白结构相关（1）。在发育的脑中有其高表达，它可以调节细胞结构和细胞分裂和迁移所需要的胞内过程（1,2）。脑成熟后，nestin的表达迅速下调，被神经纤维蛋白的表达所替代（2）。因为nestin在成熟和前体神经元和神经胶质细胞都有表达，在发育中的大脑和损伤后的大脑和脊髓也有表达，nestin已被广泛接受作为神经干/祖细胞的标记（3）。nestin的表达也在多个神经肿瘤中发现，包括胶质瘤、神经纤维瘤、星形细胞瘤，因此也被广泛接受为神经肿瘤干细胞的标记（3）。nestin的表达也在星形细胞、视网膜、心肌、胰腺和其它组织中发现（3）。所以，目前并未达成一致将其认为是神经干/祖细胞的唯一标志。

1. Michalczyk, K. and Ziman, M. (2005) Histol Histopathol 20, 665-71.
2. Giliarov, A.V. (2007) Morfologiia 131, 85-90.
3. Gilyarov, A.V. (2008) Neurosci Behav Physiol 38, 165-9.

原厂资料：

Specificity / Sensitivity

Nestin (Rat-401) Mouse mAb detects endogenous levels of nestin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with E15 rat spinal cord.

Background

Nestin is an intermediate filament family member protein that is structurally related to the neurofilament proteins (1). It is highly expressed in the developing brain, where it may help to regulate cell structure and intracellular processes required for neural cell division and migration (1,2). Upon maturation of the brain, nestin expression is quickly down-regulated and replaced by expression of the neurofilament proteins (2). Because nestin is expressed in both mature and precursor neuronal and glial cells, as well as in the developing brain and in the brain and spinal cord following damage, nestin is widely accepted as a marker of neural stem/progenitor cells (3). Expression of nestin is also found in cells from various nervous system tumors, including gliomas, neuroblastomas, astrocytomas, and is generally accepted as a marker for neural cancer stem cells (3). Nestin expression has alos been observed in astrocytes, retina, cardiac muscle, pancreas and other tissues (3). Therefore, the acceptance of nestin as an exclusive marker of neural stem/progenitor cells is not unanimous.

1. Michalczyk, K. and Ziman, M. (2005) Histol Histopathol 20, 665-71.
2. Giliarov, A.V. (2007) Morfologiia 131, 85-90.
3. Gilyarov, A.V. (2008) Neurosci Behav Physiol 38, 165-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody