Cellular Localization IF Antibody Sampler Kit

产品编号：CST-4753S 品牌：Cell Signaling Technology 原厂货号：4753S
产品分类：抗体 > 一抗 > 复合抗体试剂盒
应用分类：

包装：

1 Kit

运保温度:

–20°C

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其它组分：

β-Tubulin (9F3) Rabbit mAb 【子货号：#2128，包装：40 µl，，运保温度：–20°C】

CENP-A Antibody 【子货号：#2186，包装：40 µl，，运保温度：–20°C】

NUP98 (C39A3) Rabbit mAb 【子货号：#2598，包装：40 µl，，运保温度：–20°C】

Fibrillarin (C13C3) Rabbit mAb 【子货号：#2639，包装：40 µl，，运保温度：–20°C】

Calnexin (C5C9) Rabbit mAb 【子货号：#2679，包装：40 µl，，运保温度：–20°C】

Rab5 (C8B1) Rabbit mAb 【子货号：#3547，包装：40 µl，，运保温度：–20°C】

LC3B (D11) XP® Rabbit mAb 【子货号：#3868，包装：40 µl，，运保温度：–20°C】

Histone H3 (D1H2) XP® Rabbit mAb 【子货号：#4499，包装：40 µl，，运保温度：–20°C】

COX IV (3E11) Rabbit mAb 【子货号：#4850，包装：40 µl，，运保温度：–20°C】

描述：

在Cellular Localization IF Antibody Sampler Kit里的每一个抗体仅识别自身特异性靶蛋白以及不与其他家族成员发生交叉反应。每一个抗体都使用免疫荧光实验验证，并且如上述一样着色指定细胞器。这些蛋白的表达可能在不同细胞和组织中不同。对于每个独立试剂盒成分的specificity/sensitivity信息，请参见www.cellsignal.com 。通过人工合成相应的多肽免疫动物从而制备出兔单克隆抗体和多克隆抗体，其中多肽包括：人源β-tubulin蛋白氨基末端、人源GAPDH蛋白序列、人源calnexin蛋白序列、人源COX IV蛋白Lys29位点周围残基、人源Rab5蛋白Gly190周围残基、人源histone H3蛋白羧基末端序列、人源NUP98蛋白Pro671位点周围残基、人源fibrillarin蛋白Thr298周围残基、LC3B蛋白氨基末端残基和人源CENP-A蛋白序列。通过蛋白A和多肽亲和层析纯化抗体。细胞内定位IF Antibody Sampler Kit提供一个经济手段通过免疫荧光(IF-IC)实验去鉴定细胞器。该试剂盒包含足够一抗，且每个抗体去进行至少二十免疫荧光(IF-IC)实验或四次免疫印迹（Western blot）实验。一个蛋白质的亚细胞定位的了解可能揭示其在不同细胞进程中潜在角色。其中一点是能够确定一个标记物的亚细胞定位，该标记物是与该试剂盒中细胞器特异性抗体之一发生共定位。当这些抗体作为免疫荧光的强大工具，对于细胞裂解它们也可能被使用在免疫印迹（western blot）实验内参照。

原厂资料：

Specificity / Sensitivity

Each antibody in the Cellular Localization IF Antibody Sampler Kit recognizes only it specific target and does not cross-react with other family members. Each antibody has been validated for IF-IC and stains the organelles indicated above. Expression of these proteins may vary in different cells and tissues. Please see www.cellsignal.com for additional specificity/sensitivity information for individual kit components.

Source / Purification

Rabbit monoclonal and polyclonal antibodies are prepared by immunizing animals with a synthetic peptide corresponding to: the amino terminus of human β-tubulin, the sequence of human GAPDH, the sequence of human calnexin, residues surrounding Lys29 of human COX IV, residues surrounding Gly190 of human Rab5 protein, the carboxy-terminal sequence of human histone H3, residues surrounding Pro671 of human NUP98, residues surrounding Thr298 of human fibrillarin, residues near the amino terminus of LC3B, and human CENP-A protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Description

The Cellular Localization IF Antibody Sampler Kit provides an economical means for identification of cellular organelles by fluorescence immnuocytochemistry (IF-IC). This kit includes enough primary antibody to perform at least twenty IF-IC tests or four Western blots with each antibody.

Background

Knowledge of the subcellular location of a protein may reveal the potential role it plays in a variety of cellular processes. One can confirm the subcellular location of a marker that colocalizes with one of the organelle-specific antibodies in this kit. While these antibodies serve as powerful tools for immunofluorescence, they may also be used as western blot controls for fractionated cell lysates.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5),
150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at
–20°C. Do not aliquot the antibodies