TRA-1-60(S) (TRA-1-60(S)) Mouse mAb

• 产品编号：CST-4746P      品牌：Cell Signaling Technology       原厂货号：4746P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

TRA-1-60(S) (TRA-1-60(S))Mouse mAb 小鼠单抗识别内源性的神经氨酸酶敏感型的TRA-1-60抗原蛋白。此单克隆抗体由人类胚胎癌细胞2102Ep cl.2A6类胚胎癌细胞免疫动物产生。TRA-1-60和TRA-1-81抗体识别存在于人干细胞、胚胎癌细胞、胚胎生殖干细胞表面的抗原蛋白（1）。TRA-1-60(S)与神经氨酸酶敏感型抗原表位的蛋白聚糖相互作用（2,3），而TRA-1-81与神经氨酸酶非敏感型的相同抗原表位相互作用。近来，这种抗原蛋白被认为是足糖萼蛋白的一种形式（4）。而在生殖细胞肿瘤病人的血清中也检测到TRA-1-60的存在（5,6）。

1. Andrews, P.W. et al. (1987) Int J Androl 10, 95-104.
2. Andrews, P.W. et al. (1991) Recent Results Cancer Res 123, 63-83.
3. Badcock, G. et al. (1999) Cancer Res 59, 4715-9.
4. Schopperle, W.M. and DeWolf, W.C. (2007) Stem Cells 25, 723-30.
5. Thomson, J.A. et al. (1998) Science 282, 1145-7.
6. Marrink, J. et al. (1991) Int J Cancer 49, 368-72.

原厂资料：

Specificity / Sensitivity

TRA-1-60(S) (TRA-1-60(S)) Mouse mAb detects endogenous levels of the neuraminidase-sensitive TRA-1-60 antigen.

Source / Purification

Monoclonal antibody is produced by immunizing animals with human embryonic carcinoma 2102Ep cl.2A6 cells.

Background

TRA-1-60 and TRA-1-81 antibodies detect antigens present on the surface of human stem, teratocarcinoma, and embryonic germ cells (1). TRA-1-60(S) reacts with a neuraminidase sensitive epitope of a proteoglycan (2,3), while TRA-1-81 reacts with a neuraminidase insensitive epitope on the same antigen. Recently this antigen has been proposed to be a form of the protein podocalyxin (4). TRA-1-60 is also detected in the serum of patients with germ cell tumors (5,6).

1. Andrews, P.W. et al. (1987) Int J Androl 10, 95-104.
2. Andrews, P.W. et al. (1991) Recent Results Cancer Res 123, 63-83.
3. Badcock, G. et al. (1999) Cancer Res 59, 4715-9.
4. Schopperle, W.M. and DeWolf, W.C. (2007) Stem Cells 25, 723-30.
5. Thomson, J.A. et al. (1998) Science 282, 1145-7.
6. Marrink, J. et al. (1991) Int J Cancer 49, 368-72.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.