RKIP Antibody

• 产品编号：CST-4742S      品牌：CST       原厂货号：4742S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

RKIP Antibody兔多抗能够检测内源性RKIP总蛋白水平。该多克隆抗体是采用与人和小鼠源的RKIP蛋白序列相一致的合成肽段免疫动物而产生的。该抗体经蛋白A和肽亲和层析纯化。Raf 激酶抑制蛋白(RKIP)是磷脂酰乙醇胺结合蛋白(PEBP)家族成员，该家族与Raf-1、 MEK、MAPK相关(1)。RKIP已被证明能与Raf-1, MEK, Erk形成复合物 (2)。尽管MEK、Erk能同时结合RKIP，但是Raf-1与RKIP、RKIP与MEK之间的结合是相互排斥的。因此，RKIP可以竞争性地破坏Raf-1-MEK复合物，并有效地终止从Raf-1到MAPK的信号传递(2)。RKIP对MAPK信号转导的抑制效应，会因为RKIP的Ser153位点被PKC磷酸化而得到消除(3)。PKC对RKIP的Ser153位点的磷酸化也促进了RKIP与GRK2的结合，从而阻止了GRK2依赖的GPCR的内化(4)。RKIP也能与NF-κB通路中的分子相互作用，包括NF-κB诱导的激酶(NIK), TAK1, IKKα ，IKKβ (5)。这些相互作用能够对细胞因子诱导的NF-κB通路的活化起到拮抗作用(5)。RKIP表达的恢复与前列腺癌症转移的抑制有关，暗示RKIP可能是肿瘤转移的抑制子从而成为潜在的临床靶点，这种抑制作用是通过抑制血管侵润而实现的(6)。

1. Yeung, K. et al. (1999) Nature 401, 173-7.
2. Yeung, K. et al. (2000) Mol Cell Biol 20, 3079-85.
3. Corbit, K.C. et al. (2003) J Biol Chem 278, 13061-8.
4. Lorenz, K. et al. (2003) Nature 426, 574-9.
5. Yeung, K.C. et al. (2001) Mol Cell Biol 21, 7207-17.
6. Fu, Z. et al. (2003) J Natl Cancer Inst 95, 878-89.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Bovine

Specificity / Sensitivity

RKIP Antibody detects endogenous levels of total RKIP protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to human and mouse RKIP. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Raf kinase inhibitor protein (RKIP) is a member of the phosphatidylethanolamine-binding protein (PEBP) family that associates with Raf-1 and the MEK and MAP kinases (1). RKIP has been shown to complex with Raf-1, MEK, and ERK (2). Although MEK and ERK can simultaneously bind RKIP, the association between Raf-1 and RKIP and that of RKIP and MEK are mutually exclusive. Thus, RKIP competitively disrupts the Raf-1-MEK complex and effectively terminates signal transmission from Raf-1 to MAP kinases (2). The inhibitory effect of RKIP on MAP kinase signaling is eliminated by PKC phosphorylation of RKIP at Ser153 (3). PKC phosphorylation on Ser153 also promotes the association of RKIP with GRK2, which prevents GRK2-dependent internalization of GPCR (4). RKIP also interacts with modules of the NF-κB pathway, including NF-κB-inducing kinase (NIK), TAK1, IKKα and IKKβ (5). These interactions antagonize cytokine-induced activation of the NF-κB pathway (5). Restoration of RKIP expression is associated with the inhibition of prostate cancer metastasis, implying that RKIP may be a potential clinical target as a suppressor of tumor metastasis through inhibition of vascular invasion (6).

1. Yeung, K. et al. (1999) Nature 401, 173-7.
2. Yeung, K. et al. (2000) Mol Cell Biol 20, 3079-85.
3. Corbit, K.C. et al. (2003) J Biol Chem 278, 13061-8.
4. Lorenz, K. et al. (2003) Nature 426, 574-9.
5. Yeung, K.C. et al. (2001) Mol Cell Biol 21, 7207-17.
6. Fu, Z. et al. (2003) J Natl Cancer Inst 95, 878-89.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.