ARP3 Antibody

• 产品编号：CST-4738S      品牌：CST       原厂货号：4738S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

 ARP3 Antibody检测内源性ARP3总蛋白。该抗体不能与内源性ARP2总蛋白发生交叉反应。通过人工合成人源ARP3蛋白氨基末端相应的多肽片段去免疫动物从而制备多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。肌动蛋白的核化是从已存在的微丝上形成新的微丝，在细胞运动、分裂和胞内运输中该核化影响微丝结构。一个重要肌动蛋白核化蛋白复合物是高度保守ARP2/3复合物，是由ARP2、ARP3和ARPC1-5组成。通过核化促进因子例如 WASP/WAVE或cortactin活化之后，ARP2/3复合物促进一个已存在微丝的分支和新微丝的形成(1)。伪足的形成是降解细胞外基质细胞的小的生理过程，其能通过ARP2/3复合物肌动蛋白提高。ARP2/3蛋白与钙结合蛋白竞争，钙结合蛋白是一个actin结合蛋白并显示负性调节伪足的形成(2)。连同N-WASP一起，ARP2/3 复合物调节细胞核微丝核化，以及在转录中控制actin聚合(3)。

1. Goley, E.D. and Welch, M.D. (2006) Nat. Rev. Mol. Cell Biol. 7, 713-726.
2. Morita, T. et al. (2007) J. Biol. Chem. 282, 8454-8463.
3. Yoo, Y. et al. (2007) J. Biol. Chem. 282, 7616-7623.

原厂资料：

Specificity / Sensitivity

ARP3 Antibody detects endogenous levels of total ARP3 protein. The antibody does not cross-react with endogenous levels of total ARP2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human ARP3. Antibodies are purified using protein A and peptide affinity chromatography. 

Background

Actin nucleation, the formation of new actin filaments from existing filaments, affects actin filament structure during cell motility, division, and intracellular trafficking. An important actin nucleation protein complex is the highly conserved ARP2/3 complex, consisting of ARP2, ARP3 and ARPC1-5. The ARP2/3 complex promotes branching of an existing actin filament and formation of a daughter filament following activation by nucleation-promoting factors, such as WASP/WAVE or cortactin (1). The formation of podosomes, small cellular projections that degrade the extracellular matrix, is enhanced by ARP2/3 complex action. ARP2/3 competes with caldesmon, an actin binding protein shown to negatively affect podosome formation (2). Along with N-WASP, the ARP2/3 complex regulates nuclear actin filament nucleation and controls actin polymerization during transcription (3). 

1. Goley, E.D. and Welch, M.D. (2006) Nat. Rev. Mol. Cell Biol. 7, 713-726.
2. Morita, T. et al. (2007) J. Biol. Chem. 282, 8454-8463.
3. Yoo, Y. et al. (2007) J. Biol. Chem. 282, 7616-7623.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.