TRAF1 (1F3) Rat mAb

• 产品编号：CST-4710S      品牌：CST       原厂货号：4710S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

TRAF1 (1F3) Rat mAb 兔单抗能够检测内源性水平TRAF1总蛋白。该单克隆抗体是采用 TRAF1-GST 融合蛋白免疫动物而制备的。抗体由protein G亲和层析技术纯化。TRAFs (TNF受体相关因子)是一个多功能接头蛋白家族，能够结合表面受体并募集其它蛋白形成多蛋白信号复合物，促进细胞应答（1-3）。TRAF家族的成员有共同的羧基末端“TRAF结构域”，能够介导与其它蛋白的相互作用；许多成员同时包含氨基末端锌/环指基序。首先鉴定出的TRAF家族成员是TRAF1和TRAF2，发现它们能够与TNF受体2(TNFRII)的胞质结构域相互作用（4）。已知六个TRAF家族成员(TRAF1-6)作为细胞表面广泛受体的接头蛋白，参与调解细胞生存、增殖、分化和应激反应。

1. Arch, R.H. et al. (1998) Genes Dev. 12, 2821-2830.
2. Chung, J. Y. et al. (2002) J. Cell Sci. 115, 679-688.
3. Bradley, J.R. and Pober, J.S. (2001) Oncogene 20, 6482-6491.
4. Rothe, M. et al. (1994) Cell 78, 681-692.

原厂资料：

Specificity / Sensitivity

TRAF1 (1F3) Rat mAb detects endogenous levels of total TRAF1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a TRAF1-GST fusion protein and purified by protein G affinity chromatography.

Background

TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses (1-3). Members of the TRAF family share a common carboxy-terminal "TRAF domain" which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII) (4). The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress responses.

1. Arch, R.H. et al. (1998) Genes Dev. 12, 2821-2830.
2. Chung, J. Y. et al. (2002) J. Cell Sci. 115, 679-688.
3. Bradley, J.R. and Pober, J.S. (2001) Oncogene 20, 6482-6491.
4. Rothe, M. et al. (1994) Cell 78, 681-692.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.