hnRNP K (R332) Antibody

• 产品编号：CST-4675S      品牌：CST       原厂货号：4675S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

hnRNP K (R332) Antibody检测内源性hnRNP K总蛋白水平。通过人工合成人源hnRNP K蛋白Arg332位点周围序列相应的多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。Heterogeneous nuclear ribonucleoprotein K (hnRNP K)属于RNA结合多重蛋白复合物(hnRNP proteins)家族成员，该家族有助于pre-mRNA的加工以及mRNA从细胞核到细胞质的转运 (1-3)。hnRNP K蛋白包含三个独有的结构基序，又称KH 结构域，它们可以结合poly(C) DNA和RNA序列(4,5)。复杂的空间结构使hnRNP K蛋白促进mRNA生物合成(6)、转录调控(7)以及信号转导。实验研究发现胞质的hnRNP K蛋白在口腔鳞状细胞癌和胰腺癌中表达升高，这可能是一个潜在的预后因子(8,9)。hnRNP K还可与p53相互协调来调控靶基因的转录从而应答DNA损伤。 hnRNP K蛋白酶体降解是通过E3连接酶MDM2来介导的(10)。hnRNP K和c-Src蛋白相互作用导致hnRNP K磷酸化，从而使hnRNP K激活沉默的mRNA翻译 (11)。

1. Dreyfuss, G. et al. (1993) Annu Rev Biochem 62, 289-321.
2. Siomi, H. et al. (1994) Cell 77, 33-9.
3. Miau, L.H. et al. (1998) J Biol Chem 273, 10784-91.
4. Tomonaga, T. and Levens, D. (1995) J Biol Chem 270, 4875-81.
5. Choi, H.S. et al. (2009) Biochem Biophys Res Commun 380, 431-6.
6. Bustelo, X.R. et al. (1995) Mol Cell Biol 15, 1324-32.
7. Michelotti, E.F. et al. (1996) Mol Cell Biol 16, 2350-60.
8. Zhou, R. et al. (2010) Int J Cancer 126, 395-404.
9. Matta, A. et al. (2009) Int J Cancer 125, 1398-406.
10. Moumen, A. et al. (2005) Cell 123, 1065-78.
11. Ostareck-Lederer, A. et al. (2002) Mol Cell Biol 22, 4535-43.

原厂资料：

Specificity / Sensitivity

hnRNP K (R332) Antibody detects endogenous level of total hnRNP K protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg332 of human hnRNP K protein. Antibodies were purified by protein A and peptide affinity chromatography.

Background

Heterogeneous nuclear ribonucleoprotein K (hnRNP K) belongs to a family of RNA binding multiprotein complexes (hnRNP proteins) that facilitate pre-mRNA processing and transport of mRNA from the nucleus to cytoplasm (1-3). hnRNP K contains three unique structural motifs termed KH domains that bind poly(C) DNA and RNA sequences (4,5). Intricate architecture enables hnRNP K to facilitate mRNA biosynthesis (6), transcriptional regulation (7) and signal transduction. Cytoplasmic hnRNP K expression is increased in oral squamous cell carcinoma and pancreatic cancer and may be a potential prognostic factor (8,9). hnRNP K coordinates with p53 to regulate its target gene transcription in response to DNA damage. Proteasome degradation of hnRNP K is mediated by E3 ligase MDM2 (10). The interaction between hnRNP K and c-Src leads to hnRNP K phosphorylation, which allows for hnRNP K activation of silenced mRNA translation (11).

1. Dreyfuss, G. et al. (1993) Annu Rev Biochem 62, 289-321.
2. Siomi, H. et al. (1994) Cell 77, 33-9.
3. Miau, L.H. et al. (1998) J Biol Chem 273, 10784-91.
4. Tomonaga, T. and Levens, D. (1995) J Biol Chem 270, 4875-81.
5. Choi, H.S. et al. (2009) Biochem Biophys Res Commun 380, 431-6.
6. Bustelo, X.R. et al. (1995) Mol Cell Biol 15, 1324-32.
7. Michelotti, E.F. et al. (1996) Mol Cell Biol 16, 2350-60.
8. Zhou, R. et al. (2010) Int J Cancer 126, 395-404.
9. Matta, A. et al. (2009) Int J Cancer 125, 1398-406.
10. Moumen, A. et al. (2005) Cell 123, 1065-78.
11. Ostareck-Lederer, A. et al. (2002) Mol Cell Biol 22, 4535-43.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody