Cyclin A2 (BF683) Mouse mAb

• 产品编号：CST-4656S      品牌：CST       原厂货号：4656S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Cyclin A1 Cyclin A2 (BF683) Mouse mAb能够检测内源性水平周期蛋白A2，与其它周期蛋白及周期蛋白A1没有交叉反应。单克隆抗体是由重组人周期蛋白A免疫动物而生产的。 通过G1/S期检验点后开始DNA复制需要周期蛋白E的参与，而周期蛋白B参与了G2/M期检验点之后有丝分裂过程的启动，周期蛋白A似乎在S期和M期都起作用(1)。许多研究显示过表达周期蛋白A能够加速G1到S期的过渡，引起DNA复制；而周期蛋白A的反义DNA可以阻止DNA复制(2-4)。周期蛋白A的作用显然是进入有丝分裂的限速步骤，同时参与分裂前期的完成(5)。分裂前期的晚期，因为cdc2/cyclinB1开始活跃，周期蛋白A不再必要(5)。

1. Pagano, M. et al. (1992) EMBO. J. 11, 961-971.
2. Resnitzky, D. et al. (1995) Mol. Cell. Biol. 15, 4347-4352.
3. d'Urso, G. et al. (1990) Science 250, 786-791.
4. Zindy, F. et al. (1992) Biochem. Biophys. Res. Commun. 182, 1144-1154.
5. Furuno, N. et al. (1999) J. Cell. Biol. 147, 295-306.

原厂资料：

Specificity / Sensitivity

Cyclin A2 (BF683) Mouse mAb detects endogenous levels of cyclin A2 and does not cross-react with other cyclins or Cyclin A1 

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant human cyclin A protein. 

Background

While overcoming the G1/S checkpoint to commence DNA replication requires cyclin E, and traversing the G2/M checkpoint to initiate mitosis requires cyclin B to be present, cyclin A seems to be required for both S-phase and M-phase (1). A number of studies have described the ability of over-expressed cyclin A to accellerate the G1 to S transition causing DNA replication, and cyclin A antisense DNA can prevent DNA replication (2-4). Cyclin A availability is apparently the rate-limiting step for entry into mitosis, and cyclin A is required for completion of prophase (5). At late prophase, 

1. Pagano, M. et al. (1992) EMBO. J. 11, 961-971.
2. Resnitzky, D. et al. (1995) Mol. Cell. Biol. 15, 4347-4352.
3. d'Urso, G. et al. (1990) Science 250, 786-791.
4. Zindy, F. et al. (1992) Biochem. Biophys. Res. Commun. 182, 1144-1154.
5. Furuno, N. et al. (1999) J. Cell. Biol. 147, 295-306.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium
azide. Store at –20°C. Do not aliquot the antibody