MAD2L1 (D8A7) Rabbit mAb

• 产品编号：CST-4636S      品牌：CST       原厂货号：4636S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MAD2L1 (D8A7) Rabbit mAb 能够检测内源性 MAD2L1总蛋白。该单克隆抗体是由合成的人源的针对MAD2L1蛋白氨基末端的肽段免疫动物生产的。先于细胞分裂发生的姐妹染色单体正确分离在维持基因组完整性和避免非整倍体性和染色体不稳定性(许多肿瘤细胞的特征)的过程中至关重要。有丝分裂检验点，又称纺锤体装配检验点，监测着丝粒准确附着到纺锤体上面，抑制有丝分裂并延迟分裂后期的到来直至染色体分配到赤道板(1)。MAD2L1是有丝分裂检验点必不可少的参与者(2)。它以两种构象存在，包括打开的非激活O-MAD2形式和关闭的激活C-MAD2形式。在有丝分裂之前，MAD2L1定位于细胞质且主要以C-MAD2形式存在。在有丝分裂检验点，MAD2L1开启O-MAD2构象形式(3)。同其它的检验点蛋白一起，MAD2L1结合并抑制Cdc20，从而抑制分裂后期促进复合体(4)。当着丝粒准确附着到纺锤体以后，MAD2L1释放Cdc20，允许分裂后期促进复合体的激活，随后降降核心的有丝分裂底物，启动分裂中期-分裂后期转换(5)。

1. Wassmann, K. and Benezra, R. (2001) Curr Opin Genet Dev 11, 83-90.
2. Musacchio, A. and Salmon, E.D. (2007) Nat Rev Mol Cell Biol 8, 379-93.
3. Skinner, J.J. et al. (2008) J Cell Biol 183, 761-8.
4. Reddy, S.K. et al. (2007) Nature 446, 921-5.
5. Stegmeier, F. et al. (2007) Nature 446, 876-81.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey, Bovine, Dog

Specificity / Sensitivity

MAD2L1 (D8A7) Rabbit mAb detects endogenous levels of total MAD2L1 protein. 

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues at the amino terminus of human MAD2L1 protein. 

Background

Correct segregation of sister chromatids prior to the onset of cell division is essential to the maintenance of genetic integrity and the avoidance of aneuploidy and chromosomal instability, characteristics of many cancer cells. The mitotic checkpoint, also known as the spindle assembly checkpoint, monitors accurate attachment of kinetochores to the spindle, inhibits mitosis and delays the onset of anaphase until all chromosomes are aligned at the metaphase plate (1). MAD2L1 is an essential participant in the mitotic checkpoint (2). It exists in two conformations, including the open and inactive O-MAD2 form and the closed, active C-MAD2 form. Prior to mitosis, MAD2L1 is localized to the cytosol and exists largely in the closed, inactive form. During the mitotic checkpoint, MAD2L1 switches to the open, active conformation (3). Together with other checkpoint proteins, MAD2L1 binds to and deactivates Cdc20, thereby inhibiting the anaphase promoting complex (4). When the kinetochores are correctly attached to the spindle, MAD2L1 releases Cdc20, which allows activation of the anaphase promoting complex and subsequent degradation of key mitotic substrates and the initiation of metaphase-anaphase transition (5). 

1. Wassmann, K. and Benezra, R. (2001) Curr Opin Genet Dev 11, 83-90.
2. Musacchio, A. and Salmon, E.D. (2007) Nat Rev Mol Cell Biol 8, 379-93.
3. Skinner, J.J. et al. (2008) J Cell Biol 183, 761-8.
4. Reddy, S.K. et al. (2007) Nature 446, 921-5.
5. Stegmeier, F. et al. (2007) Nature 446, 876-81.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody