Dysbindin Antibody

• 产品编号：CST-4611S      品牌：CST       原厂货号：4611S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Dysbindin Antibody 兔多抗识别内源性的总Dysbindin蛋白。该多克隆抗体由合成的人类Dysbindin蛋白氨基酸序列对应肽段免疫动物而制成。该抗体使用多肽亲和层析纯化而得。Dysbindin或dystrobrevin结合蛋白1，是含有卷曲螺旋的蛋白，在肌肉和脑表达，能与dystrobrevin结合（1）。Dysbindin上调突触前蛋白SNAP25和synapsin I的表达，从而增加谷氨酸的释放和通过Akt信号促进神经细胞的活动。特别的，Akt磷酸化可以被dysbindin下调所抑制，被dysbindin上调所促进（2）。dysbindin无义突变导致Hermansky-Pudlak病，它是一种常染色体隐性遗传疾病，以溶酶体贮积缺陷和出血时间延长为特点。（2）编码dysbindin的基因的遗传变异与精神分裂症强烈相关，表现精神分裂症患者为前额皮层、中脑和海马的蛋白水平的降低（3,4）。

1. Benson, M.A. et al. (2001) J Biol Chem 276, 24232-41.
2. Numakawa, T. et al. (2004) Hum Mol Genet 13, 2699-708.
3. Straub, R.E. et al. (2002) Am J Hum Genet 71, 337-48.
4. Talbot, K. et al. (2004) J Clin Invest 113, 1353-63.

原厂资料：

Specificity / Sensitivity

Dysbindin Antibody detects endogenous levels of total dysbindin protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human dysbindin. Antibodies are purified by peptide affinity chromatography.

Background

Dysbindin, or dystrobrevin-binding protein 1, is a coiled-coil-containing protein expressed in muscle and brain that was identified as a binding partner of dystrobrevin (1). Dysbindin upregulates expression of the pre-synaptic proteins SNAP25 and synapsin I, thereby increasing glutamate release and promoting neuronal viability through Akt signaling. In particular, Akt phosphorylation is suppressed with downregulation of dysbindin and increased with upregulation of dysbindin (2). A nonsense mutation of dysbindin causes Hermansky-Pudlak disease, an autosomal recessive disorder characterized by lysosomal storage defects and prolonged bleeding. (2). Genetic variation in the gene encoding dysbindin is strongly associated with schizophrenia and protein levels are reduced in the prefrontal cortex, midbrain and hippocampus of brains from patients with schizophrenia (3,4).

1. Benson, M.A. et al. (2001) J Biol Chem 276, 24232-41.
2. Numakawa, T. et al. (2004) Hum Mol Genet 13, 2699-708.
3. Straub, R.E. et al. (2002) Am J Hum Genet 71, 337-48.
4. Talbot, K. et al. (2004) J Clin Invest 113, 1353-63.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.