Bik Antibody

• 产品编号：CST-4592P      品牌：Cell Signaling Technology       原厂货号：4592P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Bik Antibody能够检测内源性水平的Bik蛋白。该抗体不会与Bcl-2家族成员产生交叉反应。该多克隆抗体是采用合成的人源Bik蛋白N末端周围残基相对应的肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。Bik/Nbk（Bcl-2互作杀手/天然杀手）是一种强效的促凋亡蛋白，是Bcl-2家族的成员，该家族包括Bad、Bid、Bim、Hrk和NOXA，Bik含有一个BH3结构域，但缺乏其他保守的结构域，即BH1或BH2（1,2）。在功能上，Bik能够结合并拮抗抗凋亡的Bcl-2家族成员，包括BCL-2、Bcl-xL、病毒同源的E1B-19K和EBV-BHFR1。Bik 的BH3结构域对于它的凋亡活性，及与存活蛋白的相互作用是必不可少的（3）。Bik的磷酸化与其促凋亡活性的增加相关（4）。

1. Boyd, J. M. et al. (1995) Oncogene 11, 1921-1928.
2. Han, J. et al. (1996) Mol. Cell. Biol. 16, 5857-5864.
3. Tong, Y. et al. (2001) Mol. Cancer Ther. 1, 95-102.
4. Verma, S. et al. (2001) J. Biol. Chem. 276, 4671-4676.

原厂资料：

Specificity / Sensitivity

Bik Antibody detects endogenous levels of Bik protein. It does not cross-react with other Bcl-2 family members.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues in the amino-terminus of Bik. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Bik/Nbk (Bcl-2-interacting killer/natural born killer) is a potent pro-apoptotic protein belonging to a group of Bcl-2 family members that includes Bad, Bid, Bim, Hrk, and Noxa, containing a BH3 domain but lacking other conserved domains, BH1 or BH2 (1,2). Functionally, Bik is able to bind to and antagonize anti-apoptotic Bcl-2 family members including Bcl-2, Bcl-xL, and viral homologs E1B-19K and EBV-BHFR1. The BH3 domain of Bik is essential for its apoptotic activity and interaction with survival proteins (3). Phosphorylation of Bik is correlated with an increase in its pro-apoptotic activity (4).

1. Boyd, J. M. et al. (1995) Oncogene 11, 1921-1928.
2. Han, J. et al. (1996) Mol. Cell. Biol. 16, 5857-5864.
3. Tong, Y. et al. (2001) Mol. Cancer Ther. 1, 95-102.
4. Verma, S. et al. (2001) J. Biol. Chem. 276, 4671-4676.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.