BACH1/BRIP1 Antibody

• 产品编号：CST-4578S      品牌：CST       原厂货号：4578S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

 BACH1/BRIP1抗体可以检测内源性人源BACH1抗体。该抗体是由合成的人源的针对BACH1蛋白羧基端肽段免疫动物，利用A蛋白和多肽亲和层析方法纯化生产的。BACH1是一种DNA解螺旋酶，也称为BRIP1或FANCJ，与DNA 交联和DNA双链断裂的修复相关(1-3)。磷酸化BACH1与BRCA1的相互作用对于DNA损伤诱导的检测点信号传导是必须的(3,4)。BACH1基因最初被认为是乳腺癌的易感基因（1），在范可尼贫血中发生突变(5)，范可尼贫血是以多发性先天畸形、进行性骨髓衰竭和高肿瘤风险/易感性。与BRCA1相互作用并且在早期乳腺癌病人中存在突变提示BACH1基因可能是一个肿瘤抑制基因。

1. Cantor, S.B. et al. (2001) Cell 105, 149-60.
2. Litman, R. et al. (2005) Cancer Cell 8, 255-65.
3. Peng, M. et al. (2006) Oncogene 25, 2245-53.
4. Shiozaki, E.N. et al. (2004) Mol Cell 14, 405-12.
5. Kennedy, R.D. and D'Andrea, A.D. (2005) Genes Dev 19, 2925-40.
6. Cantor, S.B. and Guillemette, S. (2011) Future Oncol 7, 253-61.
7. Gong, Z. et al. (2010) Mol Cell 37, 438-46.

原厂资料：

Specificity / Sensitivity

BACH1/BRIP1 Antibody detects endogenous levels of human BACH1 protein. 

Source / Purification

BACH1/BRIP1 Antibody is produced by immunizing rabbits with a synthetic peptide corresponding to amino acids near the carboxy terminus of human BACH1. Antibodies are purified by Protein A and peptide affinity chromatography. 

Background

BACH1, also known as BRIP1 and FANCJ, is a DNA helicase involved in repair of DNA cross-links and double strand breaks (1-3). Interaction between phosphorylated BACH1 and BRCA1 is required for DNA damage-induced checkpoint signaling (3,4). Originally identified as a breast cancer susceptibility gene (1), the BACH1 gene is mutated in Fanconi anemia (5), a recessive disorder characterized by multiple congenital abnormalities, progressive bone marrow failure and high cancer risk/predisposition. The interaction with BRCA1 and the existence of mutations in patients with early onset breast cancer indicate that BACH1 may act as a tumor suppressor. 

1. Cantor, S.B. et al. (2001) Cell 105, 149-60.
2. Litman, R. et al. (2005) Cancer Cell 8, 255-65.
3. Peng, M. et al. (2006) Oncogene 25, 2245-53.
4. Shiozaki, E.N. et al. (2004) Mol Cell 14, 405-12.
5. Kennedy, R.D. and D'Andrea, A.D. (2005) Genes Dev 19, 2925-40.
6. Cantor, S.B. and Guillemette, S. (2011) Future Oncol 7, 253-61.
7. Gong, Z. et al. (2010) Mol Cell 37, 438-46.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody