Keratin 18 (DC10) Mouse mAb

• 产品编号：CST-4548P      品牌：Cell Signaling Technology       原厂货号：4548P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Keratin 18 (DC10) Mouse mAb识别内源性的keratin 18总蛋白。该抗体不与其它keratins发生交叉反应。使用人源PMC-42乳腺癌细胞去免疫老鼠从而制备出单克隆抗体(isotype: IgG1)。角蛋白（细胞角蛋白）是中间丝蛋白主要在上皮细胞中表达。角蛋白的异二聚体由一个酸性角蛋白（或type I角蛋白，角蛋白9-23或）和一个碱性角蛋白（type II角蛋白，角蛋白1-8）聚合形成中间丝（1.2）。角蛋白的亚型在组织-和分化的特异性表达，这使他们可做为有用的生物标志（1）。研究显示角蛋白基因突变与肌肤失调，肝脏和胰腺疾病，以及肠道炎性疾病相关（3-6）。

1. Moll, R. et al. (1982) Cell 31, 11-24.
2. Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
3. Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
4. Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
5. Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
6. Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.

原厂资料：

Specificity / Sensitivity

Keratin 18 (DC10) Mouse mAb detects endogenous levels of total keratin 18 protein. The antibody does not cross-react with other keratins.

Source / Purification

Monoclonal antibody (isotype: IgG1) is produced by immunizing mice with human PMC-42 breast carcinoma cells. 

Background

Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers (1). Mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).

1. Moll, R. et al. (1982) Cell 31, 11-24.
2. Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
3. Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
4. Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
5. Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
6. Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.