EOMES Antibody

• 产品编号：CST-4540S      品牌：CST       原厂货号：4540S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

EOMES抗体识别内源性的总EOMES蛋白。多克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与邻近人源EOMES蛋白氨基末端氨基酸序列一致。T盒家族转录因子是小鼠短尾突变体表型(T)基因产物DNA结合结构域同源物。此家族成员能具有合DNA并由转录活性。它们也具有进化保守表达方式和胚胎原中胚层发育方面的作用[1], EOMES,或Tbr2（T-box brain 2），是中胚叶形成的主要调控因子，也对滋养层细胞形成、原肠胚形成、神经发生T细胞定向分化起必需作用。EOMES基因敲出小鼠的胚胎在植入后因为不能生成滋养层细胞而很快死亡[2,3]。条件神经敲出小鼠显示在特异神经祖细胞群发育中缺失，这类细胞也称中间祖细胞(IPCs)只产生神经元[4,5]。这类细胞由处于皮质室内和室下区域的桡骨神经胶质形成。EOMES表达随细胞从桡骨神经胶质发育成IPCs的过程增加，然后随着IPCs发展为神经元而减少。最近的证据显示EOMES和IPCs可能在成人海马趾的SGZ中起神经发生作用[5]。EOMES对于记忆性T细胞和全效应器CD8+分化T细胞也是关键转录因子[6]，EOMES的表达在病毒感染和细菌感染的CD8+T细胞中被诱导，在那里充足的IL-12被产生引入急性宿主细胞反应[7]。

1. Showell, C. et al. (2004) Dev Dyn 229, 201-18.
2. Russ, A.P. et al. (2000) Nature 404, 95-9.
3. Strumpf, D. et al. (2005) Development 132, 2093-102.
4. Englund, C. et al. (2005) J Neurosci 25, 247-51.
5. Hodge, R.D. et al. (2008) J Neurosci 28, 3707-17.
6. Takayanagi, M. et al. (2003) Rheumatol Int 23, 315-8.
7. Takemoto, N. et al. (2006) J Immunol 177, 7515-9.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Human, Rat, Monkey

Specificity / Sensitivity

EOMES Antibody detects endogenous levels of total EOMES protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acid sequences at the amino terminus of human EOMES. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The T-box family of transcription factors is named for their shared homology with the DNA binding domain of the mouse brachyury (T) gene product. Members of this family bind DNA and are capable of transcriptional activation. They also have evolutionarily conserved expression patterns and roles in embryonic development, primarily mesoderm development (1). EOMES, or Tbr2 (T-box brain 2), is a master regulator of mesoderm formation that is also essential for trophoblast formation, gastrulation, neurogenesis and the differentiation of certain T cell subsets. Embryos from EOMES knock-out mice die soon after implantation due to their inability to develop a trophoblast (2,3). Conditional neural knock out mice show defects in development of a specific population of neural progenators known as Intermediate Progenator Cells (IPCs) that give rise only to neurons (4,5). These cells are formed from the radial glia in the ventricular and sub-ventricular zones of the cortex. Expression of EOMES increases as cells develop from radial glia to IPCs and then decreases as IPCs progress to neurons. Recent evidence suggests that EOMES and IPCs may also play a role neurogenesis in the adult hippocampal SGZ (5). EOMES is also a key transcription factor for memory T cells and for full effector differentiation of CD8+ T cells (6). Expression of EOMES is induced in CD8+ T cells following viral infection and bacterial infection where sufficient IL-12 has been produced to elicit acute host cell response (7).

1. Showell, C. et al. (2004) Dev Dyn 229, 201-18.
2. Russ, A.P. et al. (2000) Nature 404, 95-9.
3. Strumpf, D. et al. (2005) Development 132, 2093-102.
4. Englund, C. et al. (2005) J Neurosci 25, 247-51.
5. Hodge, R.D. et al. (2008) J Neurosci 28, 3707-17.
6. Takayanagi, M. et al. (2003) Rheumatol Int 23, 315-8.
7. Takemoto, N. et al. (2006) J Immunol 177, 7515-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.