Daxx (25C12) Rabbit mAb

• 产品编号：CST-4533S      品牌：CST       原厂货号：4533S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Daxx (25C12) Rabbit mAb兔单抗能够检测内源性水平的Daxx总蛋白。Daxx蛋白的分子量为81 kDa，已证明其实际条带在110 kDa，这是由于翻译后的超磷酸化修饰造成的（5）。该单克隆抗体是采用合成的人源Daxx蛋白Gln255周围残基相对应的肽段免疫动物而制备的。Daxx蛋白是一种广泛表达的蛋白，最初是在酵母双杂交筛选中与Fas的胞质域相互作用而被认定的。研究已发现，它能够增强Fas介导的细胞凋亡，并激活JNK信号通路（1）。然而，更多的研究表明，Daxx蛋白实际上是一种定位于早幼粒细胞白血病致癌域（PODS）的核蛋白（2,3）。至今，已经发现细胞核与CENP-C（4）、Pax3（5）、DNA甲基转移酶I（6）和染色质相关蛋白（包括组蛋白脱乙酰基酶II、H2A、H2B、H3、H4和Dek（7））的相互作用。研究已经表明，Daxx的作用是转录抑制和细胞周期调控。在小鼠中Daxx蛋白的缺失会导致广发育性细胞凋亡的胚胎致死，这说明了Daxx蛋白的作用是直接或间接地抑制细胞死亡（5）。此外，利用RNAi抑制Daxx基因的表达，确定了Daxx蛋白抗凋亡和抑制转录活性的靶点包括NF-κB和E2F-1（8）。

1. Yang, X. et al. (1997) Cell 89, 1067-1076.
2. Torii, S. et al. (1999) EMBO J. 18, 6037-6049.
3. Li, H. et al. (2000) Mol. Cell Biol. 20, 1784-1796.
4. Pluta, A.F. et al. (1998) J. Cell Sci. 111, 2029-2041.
5. Hollenbach, A.D. et al. (1999) EMBO J. 18, 3702-3711.
6. Michaelson, J.A. et al. (1999) Gene Dev. 13, 1918-1923.
7. Hollenbach, A.D. et al. (2002) J. Cell Sci. 115, 3319-3330.
8. Michaelson, J.A. and Leder, P. (2003) J. Cell Sci. 116, 345-352.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey, Bovine, Dog

Specificity / Sensitivity

Daxx (25C12) Rabbit mAb detects endogenous levels of total Daxx protein. While Daxx has a calculated MW of 81 kDa, it has been shown to run at an apparent MW of 110 kDa at least in part due to post-translational hyper-phosphorylation (5).

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to a region surrounding Gln255 of Daxx.

Background

Daxx is a ubiquitously expressed protein that was originally identified through a yeast two-hybrid screen as an interactor with the cytoplasmic domain of Fas. It was found to enhance Fas-mediated apoptosis and activate the JNK pathway (1). However, additional studies have revealed that Daxx is actually a nuclear protein localizing to promyelocytic leukemia oncogenic domains (PODs) (2,3). Nuclear interactions have since been observed with CENP-C (4), Pax3 (5), DNA methyltransferase I (6) and chromatin-associated proteins, including histone deacetylase II, H2A, H2B, H3, H4 and Dek (7). Roles for Daxx have been suggested in transcriptional repression and cell cycle control. Loss of Daxx in mice leads to embryonic lethality with extensive developmental apoptosis, suggesting a role for Daxx directly or indirectly in suppressing cell death (5). Furthermore, inhibition of Daxx expression using RNAi has confirmed Daxx to be anti-apoptotic and to repress transcriptional activity of targets including NF-κB and E2F-1 (8).

1. Yang, X. et al. (1997) Cell 89, 1067-1076.
2. Torii, S. et al. (1999) EMBO J. 18, 6037-6049.
3. Li, H. et al. (2000) Mol. Cell Biol. 20, 1784-1796.
4. Pluta, A.F. et al. (1998) J. Cell Sci. 111, 2029-2041.
5. Hollenbach, A.D. et al. (1999) EMBO J. 18, 3702-3711.
6. Michaelson, J.A. et al. (1999) Gene Dev. 13, 1918-1923.
7. Hollenbach, A.D. et al. (2002) J. Cell Sci. 115, 3319-3330.
8. Michaelson, J.A. and Leder, P. (2003) J. Cell Sci. 116, 345-352.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium
azide. Store at –20°C. Do not aliquot the antibody