MIS-R2 Antibody

• 产品编号：CST-4518S      品牌：CST       原厂货号：4518S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MIS-R2抗体识别转染水平的人源MIS-R2蛋白。多克隆抗体是通过合成肽段去免疫动物产生。这种合成肽段与邻近人源MIS-R2蛋白的Leu315氨基酸残基序列一致。Müllerian抑制底物II型受体(MIS),也称抗Müllerian荷尔蒙受体2(AMHR2), 结合一个荷尔蒙配体，指导雄性胚胎未完成发育的Müllerian管[1,2] MIS-R2是一个单一跨膜丝氨酸/苏氨酸激酶受体，属于TGF-β受体家族涉及1型受体磷酸化和Smad转录调控[3,4]。由胎儿睾丸产生的MIS会促进Müllerian管的抑制作用，否则其会在雄性胎儿中分化成子宫和输卵管[5]。对应于MIS-R2基因突变可能引起永久Müllerian管综合型2(PMD-2),一种雄性假两性同体形式，以Müllerian管抑制失败为标志[6]。MIS-R2的存在被观察到卵巢癌细胞系，对重组MIS处理起正向反应，暗示将来受体和配体都可能是重要的治疗靶点7]。

1. Visser, J.A. et al. (1995) Biochem Biophys Res Commun 215, 1029-36.
2. di Clemente, N. et al. (1994) Mol Endocrinol 8, 1006-20.
3. Teixeira, J. et al. (1996) Endocrinology 137, 160-5.
4. Gouédard, L. et al. (2000) J Biol Chem 275, 27973-8.
5. Jamin, S.P. et al. (2002) Nat Genet 32, 408-10.
6. Imbeaud, S. et al. (1996) Hum Mol Genet 5, 1269-77.
7. Masiakos, P.T. et al. (1999) Clin Cancer Res 5, 3488-99.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

MIS-R2 Antibody detects transfected levels of human MIS-R2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu315 of human MIS-R2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The type II receptor for Müllerian inhibiting substance (MIS), also known as the anti-Müllerian hormone receptor 2 (AMHR2), binds a hormone-ligand that directs the incomplete development of Müllerian ducts in male embryos (1,2). MIS-R2 is a single transmembrane serine/threonine kinase receptor of the TGF-β receptor family involved in the phosphorylation of shared type 1 receptors and Smad transcriptional regulators (3,4). MIS produced by the fetal testis promotes the regression of Müllerian ducts that would otherwise differentiate into the uterus and fallopian tubes in the male fetus (5). Corresponding MIS-R2 gene mutations can cause persistent Müllerian duct syndrome type 2 (PMDS-2), a form of male pseudohermaphroditism characterized by a failure of Müllerian duct regression (6). The presence of MIS-R2 is observed in ovarian cancer cell lines that respond positively to treatment with recombinant MIS, suggesting that both receptor and ligand may be important therapeutic tools (7).

1. Visser, J.A. et al. (1995) Biochem Biophys Res Commun 215, 1029-36.
2. di Clemente, N. et al. (1994) Mol Endocrinol 8, 1006-20.
3. Teixeira, J. et al. (1996) Endocrinology 137, 160-5.
4. Gouédard, L. et al. (2000) J Biol Chem 275, 27973-8.
5. Jamin, S.P. et al. (2002) Nat Genet 32, 408-10.
6. Imbeaud, S. et al. (1996) Hum Mol Genet 5, 1269-77.
7. Masiakos, P.T. et al. (1999) Clin Cancer Res 5, 3488-99.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody