Phospho-TAK1 (Thr184/187) (90C7) Rabbit mAb

• 产品编号：CST-4508L      品牌：CST       原厂货号：4508L
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-TAK1 (Thr184/187) (90C7) Rabbit mAb兔单抗能够检测内源性Thr184/187位点磷酸化的TAK1蛋白水平。在一些细胞裂解物中，该抗体可能与未知来源的40 kDa蛋白发生交叉反应。该单克隆抗体通过使用人源TAK1蛋白Thr184和Thr187位点周围残基相一致的磷酸化肽段免疫动物而获得。TAK1是MAPKKK家族中的成员，由TGF-β、骨形态生成蛋白和包括IL-1在内的细胞因子所激活(1,2)。在体内，TAK1的活化需要它与TAK1结合蛋白1 (TAB1)的结合，这种结合能够使TAK1蛋白磷酸化(3,4)。另一个接头蛋白TAB2能够将TAK1与TRAF6连接起来，调节IL-1刺激引起的TAK1的活化(5)。一经激活，TAK1能够磷酸化MAPK激酶MKK4和MKK3/6，MKK4和MKK3/6再分别激活JNK和p38 MAPK。另外，TAK1能够激活NF-κB通路，这是通过与TRAF6的相互作用及对NF-κB诱导激酶(NIK)的磷酸化实现的 (2)。

1. Yamaguchi, K. et al. (1995) Science 270, 2008-2011.
2. Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-256.
3. Shibuya, H. et al. (1996) Science 272, 1179-1182.
4. Sakurai, H. et al. (2000) FEBS Lett. 474, 141-145.
5. Takaesu, G. et al. (2000) Mol. Cell 4, 649-658.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Chicken, Xenopus, Zebrafish, Bovine

Specificity / Sensitivity

Phospho-TAK1 (Thr184/187) (90C7) Rabbit mAb detects endogenous levels of TAK1 only when phosphorylated at threonine 184 and threonine 187. In some cell lysates, the antibody may cross-react with a 40 kDa band of unknown origin.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a phosphopeptide corresponding to residues surrounding Thr184 and Thr187 of human TAK1.

Background

TAK1 is a mitogen-activated protein kinase kinase kinase that can be activated by TGF-β, bone morphogenetic protein and other cytokines including IL-1 (1,2). In vivo activation of TAK1 requires association with TAK1 binding protein 1 (TAB1), which triggers phosphorylation of TAK1 (3,4). Another adaptor protein, TAB2, links TAK1 with TRAF6 and mediates TAK1 activation upon IL-1 stimulation (5). Once activated, TAK1 phosphorylates MAPK kinases MKK4 and MKK3/6, which activate p38 MAPK and JNK, respectively. In addition, TAK1 activates the NF-κB pathway by interacting with TRAF6 and phosphorylating the NF-κB inducing kinase (NIK) (2).

1. Yamaguchi, K. et al. (1995) Science 270, 2008-2011.
2. Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-256.
3. Shibuya, H. et al. (1996) Science 272, 1179-1182.
4. Sakurai, H. et al. (2000) FEBS Lett. 474, 141-145.
5. Takaesu, G. et al. (2000) Mol. Cell 4, 649-658.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody