ABCG2 Antibody

• 产品编号：CST-4477S      品牌：CST       原厂货号：4477S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

ABCG2 Antibody用于检测内源性ABCG2蛋白水平。多克隆抗体通过用对应于人源ABCG2蛋白Thr180序列的合成肽免疫动物制备而得，抗体经蛋白A和肽段亲和层析纯化。ABCG2 (BCRP1/ABCP/MXR)是ATP－结合盒式转运蛋白家族的成员，它是多种化合物ATP依赖型跨膜转运蛋白，在肿瘤细胞中跟耐药性相关（1－6）。ABCG2是高度糖基化的跨膜蛋白，有六个跨膜区，这跟它的半转运功能是对应的。ABC家族蛋白通过形成同源二聚体或者异源二聚体为发挥全转运功能或半转运功能。在胎盘细胞和一些对化疗药物抵抗的肿瘤细胞系中，ABCG2高表达。在鼠类中，ABCG2 在肾脏中高表达（8）。ABCG2同时还发现在干细胞群中高表达，尤其是造血细胞和神经元干细胞，但随着分化程度的增加其表达水平下调（9-12）。

1. Doyle, L.A. and Ross, D.D. (2003) Oncogene 22, 7340-58.
2. Allen, J.D. et al. (1999) Cancer Res 59, 4237-41.
3. Doyle, L.A. et al. (1998) Proc Natl Acad Sci U S A 95, 15665-70.
4. Allikmets, R. et al. (1998) Cancer Res 58, 5337-9.
5. Miyake, K. et al. (1999) Cancer Res 59, 8-13.
6. Robey, R.W. et al. (2001) Clin Cancer Res 7, 145-52.
7. Zhou, S. et al. (2001) Nat Med 7, 1028-34.
8. Honscha, W. et al. (2000) Hepatology 31, 1296-304.
9. Scharenberg, C.W. et al. (2002) Blood 99, 507-12.
10. Islam, M.O. et al. (2005) Neurosci Res 52, 75-82.
11. Bunting, K.D. (2002) Stem Cells 20, 11-20.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey, Xenopus, Bovine, Dog

Specificity / Sensitivity

ABCG2 Antibody detects endogenous levels of total ABCG2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr180 of human ABCG2 protein, which lies within the intracellular region of ABCG2. Antibodies were purified by protein A and peptide affinity chromatography.

Background

ABCG2 (BCRP1/ABCP/MXR) is a member of the ATP-binding cassette transporter family that functions as ATP-dependent transporters for a wide variety of chemical compounds and are associated with drug-resistance in cancer cells (1-6). ABCG2 is a heavily glycosylated transmembrane protein with six transmembrane spanning regions consistent with it functioning as a half-transporter. The ABC family can exist as either full-length transporters or as half-transporters that form functional transporters through homo- or heterodimerization. High expression of ABCG2 was found in placenta as well as cell lines selected for resistance to a number of chemotherapeutic drugs, including mitoxantrone, doxorubicin, topotecan and flavopiridol. In rodents, the highest expression of ABCG2 was found in kidney (8). ABCG2 expression has also been observed in stem cell populations, particularly in hematopoietic and neuronal stem cells and is downregulated with differentiation (9-12).

1. Doyle, L.A. and Ross, D.D. (2003) Oncogene 22, 7340-58.
2. Allen, J.D. et al. (1999) Cancer Res 59, 4237-41.
3. Doyle, L.A. et al. (1998) Proc Natl Acad Sci U S A 95, 15665-70.
4. Allikmets, R. et al. (1998) Cancer Res 58, 5337-9.
5. Miyake, K. et al. (1999) Cancer Res 59, 8-13.
6. Robey, R.W. et al. (2001) Clin Cancer Res 7, 145-52.
7. Zhou, S. et al. (2001) Nat Med 7, 1028-34.
8. Honscha, W. et al. (2000) Hepatology 31, 1296-304.
9. Scharenberg, C.W. et al. (2002) Blood 99, 507-12.
10. Islam, M.O. et al. (2005) Neurosci Res 52, 75-82.
11. Bunting, K.D. (2002) Stem Cells 20, 11-20.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.