Scribble Antibody

• 产品编号：CST-4475S      品牌：CST       原厂货号：4475S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Scribble抗体检测内源性总的Scribble蛋白。多抗由合成的肽段免疫动物产生，该合成肽段对应人scribble α4蛋白的1237位甘氨酸附近残基。抗体由蛋白A和肽段亲和层析技术纯化。Scribble (Scrib) 连同细胞极性决定因素Discs Large基因 (Dlg)和幼虫巨大致死基因 (Lgl)，最初在果蝇遗传筛选中确定。果蝇突变纯合子对于这些基因有相似的表型，包括顶底极性的缺失和肿瘤组织过渡生长。这些表型相似性暗示这三种蛋白在共同通路上起功能，对于建立和保持上皮细胞顶底极性很重要（1,2）。Scribble含有许多亮氨酸富集重复和PDZ结构域，对于定位scribble到粘着连接和哺乳动物上皮细胞的底外侧的区域很重要（1,2）。Scribble据报道结合β-catenin, APC, E-cadherin 和通过对E6诱导的细胞转化很重要的短的基序来结合高危险病毒HPV的the E6蛋白（4-8）。Scribble过表达通过HPV E6/7蛋白抑制啮齿类上皮细胞转化（8）。

1. Bilder, D. and Perrimon, N. (2000) Nature 403, 676-80.
2. Bilder, D. et al. (2000) Science 289, 113-6.
3. Humbert, P.O. et al. (2008) Oncogene 27, 6888-907.
4. Sun, Y. et al. (2009) Mol Biol Cell 20, 3390-400.
5. Qin, Y. et al. (2005) J Cell Biol 171, 1061-71.
6. Navarro, C. et al. (2005) Oncogene 24, 4330-9.
7. Takizawa, S. et al. (2006) Genes Cells 11, 453-64.
8. Nguyen, M.L. et al. (2003) J Virol 77, 6957-64.

原厂资料：

Specificity / Sensitivity

Scribble Antibody detects endogenous levels of total scribble protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acid residues surrounding Gly1237 of human scribble protein. Antibodies are purified by protein A and peptide affinity chromatography。

Background

Scribble (Scrib) was originally identified in a genetic screen in Drosophila along with cell polarity determinants Discs Large (Dlg) and Lethal giant larvae (Lgl). Drosophila mutants homozygous for these genes share similar phenotypes, including the loss of apicobasal cell polarity and neoplastic tissue overgrowth. These phenotypic similarities suggest that these three proteins function in a common pathway important for establishing and maintaining apicobasal polarity in epithelial cells (1,2). Scribble contains many leucine-rich repeats and PDZ domains important for localizing scribble to adherens junctions and basolateral regions of mammalian epithelial cells (3). Scribble reportedly binds β-catenin, APC, E-cadherin and the E6 protein from high-risk virus type of HPV through a short motif important for E6-induced cell transformation (4-8). Overexpression of scribble inhibits transformation of rodent epithelial cells by HPV E6/7 proteins (8).

1. Bilder, D. and Perrimon, N. (2000) Nature 403, 676-80.
2. Bilder, D. et al. (2000) Science 289, 113-6.
3. Humbert, P.O. et al. (2008) Oncogene 27, 6888-907.
4. Sun, Y. et al. (2009) Mol Biol Cell 20, 3390-400.
5. Qin, Y. et al. (2005) J Cell Biol 171, 1061-71.
6. Navarro, C. et al. (2005) Oncogene 24, 4330-9.
7. Takizawa, S. et al. (2006) Genes Cells 11, 453-64.
8. Nguyen, M.L. et al. (2003) J Virol 77, 6957-64.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.