Bif-1 Antibody

• 产品编号：CST-4467S      品牌：CST       原厂货号：4467S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Bif-1 Antibody能识别内源性的Bif-1总蛋白。该多克隆抗体是采用合成的人Bif-1 蛋白 Gly129周围的残基相对应的肽段免疫动物制备而得。抗体通过蛋白A以及多肽亲和色谱法纯化。Bif-1/SH3GLB1/Endophilin-B1，是endophilin B家族的成员之一，最初通过酵母双杂交筛选作为Bax结合蛋白被发现（1,2）。Bif-1与其他Bcl-2家族成员没有显著的同源性，在氨基末端有一个与膜动力学有关的BAR结构域，在羧基末端有一个SH3结构域。Bif-1的过表达会促进Bax构象变化和细胞凋亡（2,3）。同样地，Bif-1的缺乏会抑制Bax和Bak活化、细胞色素c的释放和半胱天冬酶的活化（3）。Bif-1主要集中于细胞内的细胞器的膜上，与膜动力学有关，包括自噬过程中的膜动力学。Bif-1直接与UVRAG结合，与Beclin-1形成复合物，从而使自噬体成熟所需要的PI3激酶类III级/Vps34的活力增加（4）。GSK-3β的抑制，就像营养匮乏的时候一样，会引起Bif-1表达增加，促进自噬性细胞死亡（5）。研究表明，Bif-1的缺乏会促进肿瘤形成，并且在一些癌症中检测到了Bif-1表达的减少（6-11）。

1. Pierrat, B. et al. (2001) Genomics 71, 222-34.
2. Cuddeback, S.M. et al. (2001) J Biol Chem 276, 20559-65.
3. Takahashi, Y. et al. (2005) Mol Cell Biol 25, 9369-82.
4. Takahashi, Y. et al. (2007) Nat Cell Biol 9, 1142-51.
5. Yang, J. et al. (2010) J Cell Sci 123, 861-70.
6. Coppola, D. et al. (2011) Pancreas 40, 433-7.
7. Coppola, D. et al. (2008) Cancer 113, 2665-70.
8. Coppola, D. et al. (2008) Clin Genitourin Cancer 6, 117-21.
9. Kim, S.Y. et al. (2008) Pathology 40, 553-7.
10. Lee, J.W. et al. (2006) Pathology 38, 312-5.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey, Bovine, Pig

Specificity / Sensitivity

Bif-1 Antibody recognizes endogenous levels of total Bif-1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly129 of human Bif-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Bif-1/SH3GLB1/Endophilin-B1 is a member of the endophilin B family that was originally identified as a Bax binding protein through yeast two-hybrid screening (1,2). Bif-1 does not have significant homology to other Bcl-2 family members, but rather contains an N-terminal Bin-Amphiphysin-Rvs (BAR) domain, typically involved in membrane dynamics, and a C-terminal SH3 domain. Overexpression of Bif-1 promotes Bax conformational change and apoptosis (2,3). Likewise, loss of Bif-1 inhibits Bax and Bak activation, cytochrome c release, and caspase activation (3). Bif-1 is localized to membranes of intracellular organelles and has been suggested to play a role in membrane dynamics, including that during autophagy. Bif-1 directly binds to UVRAG, forming a complex with Beclin-1, resulting in increased PI3-kinase class III/Vps34 activity required for autophagosome maturation (4). Inhibition of GSK-3β, as seen during nutrient deprivation, results in increased expression of Bif-1, and can contribute to autophagic cell death (5). Research studies have shown that loss of Bif-1 promotes tumorigenesis, and decreased expression of Bif-1 has been noted in several cancer types (6-11).

1. Pierrat, B. et al. (2001) Genomics 71, 222-34.
2. Cuddeback, S.M. et al. (2001) J Biol Chem 276, 20559-65.
3. Takahashi, Y. et al. (2005) Mol Cell Biol 25, 9369-82.
4. Takahashi, Y. et al. (2007) Nat Cell Biol 9, 1142-51.
5. Yang, J. et al. (2010) J Cell Sci 123, 861-70.
6. Coppola, D. et al. (2011) Pancreas 40, 433-7.
7. Coppola, D. et al. (2008) Cancer 113, 2665-70.
8. Coppola, D. et al. (2008) Clin Genitourin Cancer 6, 117-21.
9. Kim, S.Y. et al. (2008) Pathology 40, 553-7.
10. Lee, J.W. et al. (2006) Pathology 38, 312-5.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody