ARK5 Antibody

• 产品编号：CST-4458S      品牌：CST       原厂货号：4458S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

ARK5 Antibody用于检测内源性ARK5总蛋白水平。多克隆抗体通过用对应于人源ARK5蛋白序列的合成肽免疫动物制备而得，抗体经蛋白A和肽段亲和层析纯化。AMP激活的蛋白激酶包括一族丝氨酸/苏氨酸蛋白激酶家族，它们从酵母到植物，再到动物中都高度保守，在能量平衡的调节中发挥关键的作用（1）。AMPKα1, AMPKα2, MELK 和 SNAR是该蛋 白家族的催化亚基（2）。最近，AMPK相关蛋白激酶5 ARK5被发现，它跟SNARK蛋白的催化结构域有84%的同源性（2）。体外试验中，AKT可以磷酸化ARK5的600位丝氨酸。该位点的磷酸化可以 激活ARK5，这反过来会增加SAMS肽段的磷酸化水平，SAMS是AMPK的共同底物（2）。体内实验显示Akt激活的ARK5对于细胞在糖饥饿状态下的生存是很重要的（2）。此外，一些研究同时也发现 了ARK5和肿瘤侵袭间的联系（3,4）。ARK5的过表达会导致肿瘤的快速生长（3）。例如，在胰腺肿瘤细胞PANC-1过表达ARK5会加速其在肝脏中的转移（4）。

1. Hardie, D.G. (2004) J. Cell Sci. 117, 5479-87.
2. Suzuki, A. et al. (2003) J. Biol. Chem. 278, 48-53.
3. Suzuki, A. et al. (2004) Mol. Cell Biol. 24, 3526-35.
4. Kusakai, G. et al. (2004) J. Exp. Clin. Cancer Res. 23, 263-8.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Bovine

Specificity / Sensitivity

ARK5 Antibody detects endogenous levels of total ARK5 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human ARK5. Antibodies are purified by protein A and peptide affinity chromatography.

Background

AMP-activated protein kinases (AMPKs) constitute a serine/threonine protein kinase family, which is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis (1). AMPKα1, AMPKα2, MELK and SNARK are the catalytic subunits in the family (2). Recently, AMPK-related protein kinase ARK5 was identified, which shares 84% similarity with the sequence of the catalytic domain of SNARK (2). In vitro, Akt phosphorylates ARK5 at Ser600. This phosphorylation activates ARK5, which in turn results in the increased phosphorylation of the SAMS peptide, an AMPK consensus substrate (2). In vivo experiments showed that Akt-activated ARK5 is critical for the survival of cells under glucose starvation (2). Furthermore, studies also linked ARK5 to tumor invasion (3, 4). Overexpressed ARK5 leads to higher tumor growth rate (3). For example, the overexpression of ARK5 in pancreatic tumor cell line PANC-1 significantly elevates its metastasis in the liver (4).

1. Hardie, D.G. (2004) J. Cell Sci. 117, 5479-87.
2. Suzuki, A. et al. (2003) J. Biol. Chem. 278, 48-53.
3. Suzuki, A. et al. (2004) Mol. Cell Biol. 24, 3526-35.
4. Kusakai, G. et al. (2004) J. Exp. Clin. Cancer Res. 23, 263-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.