TWEAK Antibody

• 产品编号：CST-4437S      品牌：CST       原厂货号：4437S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

TWEAK抗体能够检测重组和转染的TWEAK蛋白总体水平。此多克隆抗体是通过合成人源对应的Tweak His110位点周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。TWEAK (TNFSF12/Apo-3L)是细胞因子TNF超家族中的一员，通常参与到免疫调控，炎症和凋亡过程中(1,2)。TWEAK mRNA在许多组织和细胞系中都有表达，但是在心脏，脑组织，骨骼肌和免疫系统中具有稍高的表达 (1)。 与其它的家族成员相似，TWEAK是II型跨膜蛋白，也能被蛋白酶酶解形成可溶形式的细胞因子。可溶性的TWEAK在一些细胞系中是一个弱的凋亡诱导因子(1)。TWEAK的受体叫做TWEAKR或者 成纤维细胞诱导生长因子14(Fn14), 是相对较小的一个TNF受体家族(3)。TWEAK信号通路与凋亡，细胞增殖，细胞迁移，血管再生以及炎症反应有关系(4)。最近的研究表明在自身免疫，癌症和血管受伤中TWEAK和其受体是潜在的治疗靶标(5-8)。

1. Chicheportiche, Y. et al. (1997) J Biol Chem 272, 32401-10.
2. Marsters, S.A. et al. (1998) Curr Biol 8, 525-8.
3. Wiley, S.R. et al. (2001) Immunity 15, 837-46.
4. Wiley, S.R. and Winkles, J.A. (2003) Cytokine Growth Factor Rev 14, 241-9.
5. Desplat-Jégo, S. et al. (2005) Clin Immunol 117, 15-23.
6. Mueller, A.M. et al. (2005) J Neuroimmunol 159, 55-65.
7. Winkles, J.A. et al. (2006) Cancer Lett 235, 11-7.
8. Ortiz, A. et al. (2009) Cytokine Growth Factor Rev 20, 251-8.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Monkey

Specificity / Sensitivity

TWEAK Antibody detects recombinant and transfected levels of TWEAK protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His110 of human Tweak protein. Antibodies were purified by protein A and peptide affinity chromatography.

Background

TWEAK (TNFSF12/Apo-3L) is a member of the TNF superfamily of cytokines that are typically involved in immune regulation, inflammation, and apoptosis (1,2). TWEAK mRNA is expressed in a variety of tissues and cell lines, with higher levels observed in the heart, brain, skeletal muscle and within the immune system (1). Like other family members TWEAK is a type II transmembrane protein that can also be proteolytically processed to form a soluble cytokine. Soluble TWEAK is a weak inducer of apoptosis in some cell lines (1). The receptor for TWEAK, known as TWEAKR or fibroblast growth factor inducible 14 (Fn14), is a relatively small member of the TNF receptor family (3). TWEAK signaling has been associated with apoptosis, proliferation, migration, angiogenesis, and inflammation (4). Recent studies have suggested some therapeutic potential of TWEAK and its receptor signaling in regards to autoimmunity, cancer, and vascular injury (5-8).

1. Chicheportiche, Y. et al. (1997) J Biol Chem 272, 32401-10.
2. Marsters, S.A. et al. (1998) Curr Biol 8, 525-8.
3. Wiley, S.R. et al. (2001) Immunity 15, 837-46.
4. Wiley, S.R. and Winkles, J.A. (2003) Cytokine Growth Factor Rev 14, 241-9.
5. Desplat-Jégo, S. et al. (2005) Clin Immunol 117, 15-23.
6. Mueller, A.M. et al. (2005) J Neuroimmunol 159, 55-65.
7. Winkles, J.A. et al. (2006) Cancer Lett 235, 11-7.
8. Ortiz, A. et al. (2009) Cytokine Growth Factor Rev 20, 251-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.