CARD11 (1D12) Rabbit mAb

• 产品编号：CST-4435S      品牌：CST       原厂货号：4435S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CARD11 (1D12) Rabbit mAb 能够检测内源CARD11蛋白总体水平。此单克隆抗体是通过合成对应的CARD11 thr175位点周围的肽段来免疫动物而获得。CARD11/Carma1/Bimp3属于MAGUK (膜相关的鸟苷酸激酶) 家族，其典型的功能是为多蛋白复合体的组装提供分子支架(1,2)。MAGUK家族成员包含一个SH3域、一个PDZ 域和一个GuK域并与鸟苷酸激酶同源。另外，CARD11包含一个N-末端CARD 域(蛋白酶级联反应招募域)。此结构域在与很多含有CARD域的蛋白相互作用来调控凋亡和NF-κB激活过程中发挥了重要的作用。CARD11主要在淋巴细胞中表达 (1,2)并与Bcl10的CARD域相关。过表示时，CARD11 导致了 Bcl10的磷酸化和NF-κB的激活(1,2)。CARD11构成上与脂质有关在功能上能够招募Bcl10和MALT1并引起 IKKs的磷酸化(3,4)。一些利用遗传学方法破坏CARD11或采用显性负性突变的研究表明，它在NF-κB的激活和淋巴细胞的信号中起了重要的作用(4-7)。

1. Bertin, J. et al. (2001) J. Biol. Chem. 276, 11877–11882.
2. Gaide, O. et al. (2001) FEBS Lett. 496, 121-127.
3. Stilo, R. et al. (2004) J. Biol. Chem. 279, 34323-34331.
4. Wang, D. et al. (2002) Nat. Immunol. 3, 830-835.
5. Jun, J.E. et al. (2003) Immunity 18, 751-762.
6. Hara, H. et al. (2003) Immunity 18, 763-775.
7. Gaide, O. et al. (2002) Nat. Immunol. 3, 836-843.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat, Monkey

Specificity / Sensitivity

CARD11 (1D12) Rabbit mAb detects endogenous levels of total CARD11 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding threonine 175 of CARD11.

Background

CARD11/Carma1/Bimp3 belongs to the MAGUK (membrane-associated guanylate kinase) family that typically function as molecular scaffolds in the assembly of multiprotein complexes (1,2). MAGUK family members contain an SH3 domain, a PDZ domain and a GuK domain homologous to guanylate kinase. In addition, CARD11 contains an amino-terminal CARD domain (caspase recruitment domain). This domain plays an important role in forming interactions with a number of proteins containing CARD domains that are involved in regulating apoptosis and NF-κB activation. CARD11 is predominately expressed in lymphocytes (1,2) and associates with the CARD domain of Bcl10. When overexpressed, CARD11 leads to the phosphorylation of Bcl10 and activation of NF-κB (1,2). CARD11 is constitutively associated with lipid rafts and is thought to function by recruiting Bcl10 and MALT1 and triggering the phosphorylation of IKKs (3,4). Several studies using the genetic disruption of CARD11 or dominant-negative mutations have demonstrated that it plays a critical role in NF-κB activation and lymphocyte signaling (4-7).

1. Bertin, J. et al. (2001) J. Biol. Chem. 276, 11877–11882.
2. Gaide, O. et al. (2001) FEBS Lett. 496, 121-127.
3. Stilo, R. et al. (2004) J. Biol. Chem. 279, 34323-34331.
4. Wang, D. et al. (2002) Nat. Immunol. 3, 830-835.
5. Jun, J.E. et al. (2003) Immunity 18, 751-762.
6. Hara, H. et al. (2003) Immunity 18, 763-775.
7. Gaide, O. et al. (2002) Nat. Immunol. 3, 836-843.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.