FTH1 (D1D4) Rabbit mAb

• 产品编号：CST-4393S      品牌：CST       原厂货号：4393S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

FTH1(D1D4)兔单克隆抗体识别内源性的FTH1总蛋白水平。该单克隆抗体用与人类FTH1蛋白中羧基末端附近的氨基酸序列对应的人工合成肽段免疫动物制成。铁蛋白（FTU）是一个广泛分布而且高度保守的蛋白质，在铁平衡中发挥很大作用，以无毒和生物可适应的形式完成铁的吸收和储存（1）。组装好的铁蛋白分子，经常被认为呈纳米小笼状，可以贮存4500个铁原子（2,3）。它形成一个～450 kDa的全酶，包括由两种多肽链（铁蛋白重链和铁蛋白轻链，各有独特作用）组成的24个亚基。铁蛋白重链催化铁储存的第一步，Fe（II）的氧化；铁蛋白轻链促进水合铁的成核，允许Fe（III）的储存（4）。除了缓冲铁以外，重链还可以增强胸腺嘧啶的生物合成（5）。血清铁蛋白水平是反映机体铁储存的指标。血清铁蛋白是贫血指标中最敏感的。血清铁蛋白水平在炎症、恶性疾病和铁超载疾病中明显上升（6）。研究发现铁蛋白缺陷与多种神经退行性疾病相关（7）。

1. Munro, H.N. et al. (1988) Ann N Y Acad Sci 526, 113-23.
2. Theil, E.C. (2004) Annu Rev Nutr 24, 327-43.
3. Theil, E.C. et al. (2006) J Biol Inorg Chem 11, 803-10.
4. Treffry, A. et al. (1992) FEBS Lett 302, 108-12.
5. Oppenheim, E.W. et al. (2001) J Biol Chem 276, 19855-61.
6. Tarng, D.C. (2009) Nat Clin Pract Nephrol 5, 66-7.
7. Fischer, P. et al. (1997) Life Sci 60, 2273-8.

原厂资料：

Specificity / Sensitivity

FTH1 (D1D4) Rabbit mAb recognizes endogenous levels of total FTH1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human FTH1 protein.

Background

Ferritin (FTH) is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis by sequestering and storing iron in a non-toxic and bioavailable form (1). The assembled ferritin molecule, often referred to as a nanocage, can store up to 4,500 atoms of iron (2,3). It forms a holoenzyme of ~450 kDa, consisting of 24 subunits made up of two types of polypeptide chains: ferritin heavy chain and ferritin light chain, each having unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe(II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe(III) (4). In addition to iron buffering, heavy chain ferritin also enhances thymidine biosynthesis (5). Serum ferritin levels serve as an indicator of the amount of iron stored in the body. Serum ferritin is the most sensitive test for anaemia. The level of serum ferritin is markedly elevated in inflammation, malignancy, and iron overload disorders (6). Research studies have found that defects in ferritin proteins are also associated with several neurodegenerative diseases (7).

1. Munro, H.N. et al. (1988) Ann N Y Acad Sci 526, 113-23.
2. Theil, E.C. (2004) Annu Rev Nutr 24, 327-43.
3. Theil, E.C. et al. (2006) J Biol Inorg Chem 11, 803-10.
4. Treffry, A. et al. (1992) FEBS Lett 302, 108-12.
5. Oppenheim, E.W. et al. (2001) J Biol Chem 276, 19855-61.
6. Tarng, D.C. (2009) Nat Clin Pract Nephrol 5, 66-7.
7. Fischer, P. et al. (1997) Life Sci 60, 2273-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.