ATP2A2/SERCA2 Antibody

• 产品编号：CST-4388S      品牌：CST       原厂货号：4388S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

ATP2A2/SERCA2抗体识别内源性的全部ATP2A2/SERCA2蛋白。该多克隆抗体用与人类ATP2A2/SERCA2蛋白Leu32位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。ATP2A2（SERCA2）钙泵是肌浆和内质网Ca2+-ATP酶中的一种，可调节钙跨细胞膜转运到细胞内（1）。目前已分离多个亚型，ATP2A2a(SERCA2a)主要在肌肉细胞肌浆网中发现，ATP2A2b (SERCA2b)在更普遍分布于大多数细胞的内质网（2）。一个含有截断型羧基区域的亚型（ATP2A2c）在上皮细胞和造血干细胞系表达，并可能参与了单核细胞分化（3）。ATP2A2 (SERCA2)的转录后修饰，包括磷酸化和酪氨酸硝化，可以调节Ca2+-ATP酶活性和钙的转运（4,5）。ATP2A2 (SERCA2)基因突变可导致Darier病，一种以皮肤黑变，角化型丘疹或头部和躯干部皮疹为特点的皮肤病（6）。

1. Vangheluwe, P. et al. (2005) Cell Calcium 38, 291-302.
2. de Smedt, H. et al. (1991) J Biol Chem 266, 7092-5.
3. Gélébart, P. et al. (2003) Biochem Biophys Res Commun 303, 676-84.
4. Hawkins, C. et al. (1995) Mol Cell Biochem 142, 131-8.
5. Viner, R.I. et al. (1999) Biochem J 340 ( Pt 3), 657-69.
6. Sakuntabhai, A. et al. (1999) Hum Mol Genet 8, 1611-9.

原厂资料：

Specificity / Sensitivity

ATP2A2/SERCA2 Antibody detects endogenous levels of total ATP2A2/SERCA2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence around Leu32 of human ATP2A2/SERCA2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The ATP2A2 (SERCA2) calcium pump is one of several sarcoplasmic and endoplasmic reticulum Ca2+-ATPases responsible for regulating calcium transport across intracellular membranes (1). Multiple isoforms have been isolated, with ATP2A2a (SERCA2a) found predominantly in the sarcoplasmic reticulum of muscle cells and ATP2A2b (SERCA2b) more ubiquitously expressed in the endoplasmic reticulum of most cell types (2). An isoform containing a truncated carboxy region (ATP2A2c) is expressed in epithelial and hematopoietic cell lines and may be involved in monocyte differentiation (3). Post-translational modification of ATP2A2 (SERCA2), including phosphorylation and tyrosine nitration, modify Ca2+ -ATPase activity and calcium transport (4,5). Mutation in the corresponding ATP2A2 (SERCA2) gene results in Darier disease, a skin disorder characterized by the presence of dark, keratotic papules or rash found on the head and torso (6).

1. Vangheluwe, P. et al. (2005) Cell Calcium 38, 291-302.
2. de Smedt, H. et al. (1991) J Biol Chem 266, 7092-5.
3. Gélébart, P. et al. (2003) Biochem Biophys Res Commun 303, 676-84.
4. Hawkins, C. et al. (1995) Mol Cell Biochem 142, 131-8.
5. Viner, R.I. et al. (1999) Biochem J 340 ( Pt 3), 657-69.
6. Sakuntabhai, A. et al. (1999) Hum Mol Genet 8, 1611-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody