Phospho-(Ser/Thr) PKD Substrate Antibody

• 产品编号：CST-4381S      品牌：CST       原厂货号：4381S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-(Ser/Thr) PKD Substrates Antibody识别包含-3位上有精氨酸和-5位上有亮氨酸的磷酸化丝氨酸/苏氨酸残基的肽段或蛋白，最好在-1位上还有脯氨酸。此抗体可能与包含-3位精氨酸和-5位疏水或碱性氨基酸的肽段或蛋白有交叉反应。（美国专利号：6,441,140、6,982,318、7,259,022、7,344,714；U.S.S.N. 11,484,485；及所有国外相应专利）该多克隆抗体用合成的磷酸化PKD底物肽段免疫动物制备。该抗体使用蛋白A和蛋白亲和层析纯化。Protein kinase D1 (PKD1)，也称为PKCμ，是蛋白激酶D家族的一员。PKD成员是丝氨酸/苏氨酸蛋白激酶，涉及多种细胞内功能包括细胞生长和存活、高尔基体组装和免疫反应等（1）。目前对PKD自然底物的信息了解有限（2,3）。该酶识别并磷酸化一致序列：LXRXXS/T。Phospho-(Ser/Thr) PKD Substrate Antibody识别含共同基序磷酸化的PKD，为PKD目标物的发现和鉴定提供了强大的工具，也为潜在激酶调节者的高通量药物筛选提供了强大的工具。

1. Rykx, A. et al. (2003) FEBS Lett 546, 81-6.
2. Nishikawa, K. et al. (1997) J Biol Chem 272, 952-60.
3. Iglesias, T. et al. (2000) J Biol Chem 275, 40048-56.

原厂资料：

Specificity / Sensitivity

Phospho-(Ser/Thr) PKD Substrates Antibody detects peptides and proteins containing a phospho-Ser/Thr residue with arginine at the -3 position and leucine at the -5 position, prefering proline at the -1 position within the motif. The antibody may cross-react with some peptides and proteins containing an arginine residue at -3 position and a hydrophobic or basic residue at the -5 position. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phospho-PKD substrate peptide. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Protein kinase D1 (PKD1), also called PKCμ, is one member of the protein kinase D family. PKD members are serine/threonine protein kinases implicated in very diverse cellular functions including cell growth and survival, Golgi organization and immune response (1). The information about the natural substrates of PKD is limited (2,3). The enzyme recognizes and phosphorylates the following consensus sequence: LXRXXS/T. Phospho-(Ser/Thr) PKD Substrate Antibody recognizes phosphorylated PKD substrates at their consensus motif, providing a powerful new tool for PKD target discovery and characterization as well as HTS drug screening for potential kinase regulators.

1. Rykx, A. et al. (2003) FEBS Lett 546, 81-6.
2. Nishikawa, K. et al. (1997) J Biol Chem 272, 952-60.
3. Iglesias, T. et al. (2000) J Biol Chem 275, 40048-56.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.