IRAK2 Antibody

• 产品编号：CST-4367P      品牌：Cell Signaling Technology       原厂货号：4367P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

IRAK2抗体能够检测内源性IRAK2蛋白总体水平。此抗体不与其它家族成员交叉反应。此多克隆抗体是通过合成人源对应的IRAK2 C-末端周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。细胞白介素1(IL-1)受体相关激酶(IRAK)是丝氨酸/酪氨酸特异性激酶，它通过IL-1诱导的方式与IL-1受体发生共沉淀(1)。 哺乳动物的IRAK家族有四个成员(IRAK1, IRAK2, IRAK3/IRAK-M and IRAK4)。IL-1结合到IL-1的I类受体(IL-1RI)而促进形成包含IL-1RI, AcP, MyD88和IRAKs的复合体(2)。IRAK在IL-1刺激后能够在短时间内自磷酸化。接着IRAK从IL-1RI复合体上解离，泛素化并与两个膜结合蛋白 TAB2 和TRAF6结合。IRAK-TRAF6-TAB2复合体释放到细胞质中并激活蛋白激酶的级联反应，包括TAK1、IKKs 和应激活化激酶 (3)。与IRAK1和 IRAK4不同, IRAK2 和IRAK-M并不具有重要的激酶活性，尽管他们在过表达后也能激活NF-κB(4,5)。针对IRAK2反义核酸链能抑制IL-1介导的NF-κB的激活(6)。

1. Dinarello, C.A. (1996) Blood 87, 2095-147.
2. Takaesu, G. et al. (2001) Mol Cell Biol 21, 2475-84.
3. Janssens, S. and Beyaert, R. (2003) Mol Cell 11, 293-302.
4. Wesche, H. et al. (1999) J Biol Chem 274, 19403-10.
5. Muzio, M. et al. (1997) Science 278, 1612-5.
6. Guo, F. et al. (1999) Inflammation 23, 535-43.

原厂资料：

Specificity / Sensitivity

IRAK2 Antibody detects endogeneous levels of total IRAK2 protein. Cross-reactivity was not detected with other family members.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxy terminus of human IRAK2. Antibodies were purified by protein A and peptide affinity chromatography.

Background

Interleukin-1 (IL-1) receptor-associated kinase (IRAK) is a serine/threonine-specific kinase that can be coprecipitated in an IL-1-inducible manner with the IL-1 receptor (1). The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2, IRAK3/IRAK-M and IRAK4). The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1RI, AcP, MyD88 and IRAKs (2). IRAK undergoes autophosphorylation shortly after IL-1 stimulation. The subsequent events involve IRAK dissociation from the IL-1RI complex, its ubiquitination and its association with two membrane-bound proteins: TAB2 and TRAF6. The resulting IRAK-TRAF6-TAB2 complex is then released into the cytoplasm and activates protein kinase cascades, which include TAK1, IKKs and the stress-activated kinases (3).Unlike IRAK1 and IRAK4, IRAK2 and IRAK-M do not have significant kinase activity although they can still activate NF-κB when overexpressed (4,5). Antisense oligonucleotide depletion of IRAK2 can inhibit IL-1 mediated NF-κB activation (6).

1. Dinarello, C.A. (1996) Blood 87, 2095-147.
2. Takaesu, G. et al. (2001) Mol Cell Biol 21, 2475-84.
3. Janssens, S. and Beyaert, R. (2003) Mol Cell 11, 293-302.
4. Wesche, H. et al. (1999) J Biol Chem 274, 19403-10.
5. Muzio, M. et al. (1997) Science 278, 1612-5.
6. Guo, F. et al. (1999) Inflammation 23, 535-43.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody