Phospho-RIP2 (Ser176) Antibody

• 产品编号：CST-4364S      品牌：Cell Signaling Technology       原厂货号：4364S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-RIP2 (Ser176) Antibody只能检测内源的在Ser176位点磷酸化的RIP2蛋白。背景中有一条未知来源并且分子量稍高的条带。此多克隆抗体是通过合成人源对应的RIP2 Ser176位点周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。受体相互作用蛋白2 (RIP2)是一个丝氨酸/苏氨酸激酶，具有C-末端的级联反应激活区和招募区(CARD)。连接RIP2 与肿瘤坏死因子受体(TNFR)引起了NF-kB信号的激活并诱导了凋亡(1-3)。将巨噬细胞暴露到细菌细胞壁成分如LPS中能够诱导 RIP2的表达。RIP2缺失的小鼠模型表明此激酶在天然免疫和获得性免疫中整合并传导的信号(4,5)。Serine 176位点是调控自磷酸化的位点，它能用来监控 RIP2的活性状态(6)。

1. Inohara, N. et al. (1998) J Biol Chem 273, 12296-300.
2. McCarthy, J.V. et al. (1998) J Biol Chem 273, 16968-75.
3. Thome, M. et al. (1998) Curr Biol 8, 885-8.
4. Chin, A.I. et al. (2002) Nature 416, 190-4.
5. Kobayashi, K. et al. (2002) Nature 416, 194-9.
6. Dorsch, M. et al. (2006) Cell. Signal. 18, 2223-2229.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat

Specificity / Sensitivity

Phospho-RIP2 (Ser176) Antibody detects endogenous levels of RIP2 only when phosphorylated at Ser176. Background bands of unknown origin and higher molecular weight are detected.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a phosphopeptide corresponding to residues surrounding Ser176 of human RIP2. Antibodies are purified by protein A and affinity chromatography.

Background

Receptor Interacting Protein 2 (RIP2) is a serine/threonine kinase with a carboxy-terminal caspase activation and recruitment domain (CARD). Association of RIP2 with the tumor necrosis factor receptor (TNFR) causes activation of NF-kB and induction of apoptosis (1-3). Expression of RIP2 is induced in macrophages upon exposure to bacterial cell wall components such as LPS. RIP2-deficient mouse models demonstrate that this kinase integrates and transduces signals for both the innate and adaptive immune system (4,5).Serine 176 is a regulatory autophosphorylation site and can be used to monitor the activation state of RIP2 (6).

1. Inohara, N. et al. (1998) J Biol Chem 273, 12296-300.
2. McCarthy, J.V. et al. (1998) J Biol Chem 273, 16968-75.
3. Thome, M. et al. (1998) Curr Biol 8, 885-8.
4. Chin, A.I. et al. (2002) Nature 416, 190-4.
5. Kobayashi, K. et al. (2002) Nature 416, 194-9.
6. Dorsch, M. et al. (2006) Cell. Signal. 18, 2223-2229.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.