IRAK4 Antibody

• 产品编号：CST-4363S      品牌：CST       原厂货号：4363S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

IRAK4 抗体能够检测内源性IRAK4蛋白总体水平。此抗体不与其它家族成员交叉反应。此多克隆抗体是通过合成人源对应的IRAK4 lys41位点周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。细胞白介素1(IL-1)受体相关激酶(IRAK)是丝氨酸/酪氨酸特异性激酶，它通过IL-1诱导的方式与IL-1受体发生共沉淀(1)。 哺乳动物的IRAK家族有四个成员(IRAK1, IRAK2, IRAK3/IRAK-M and IRAK4)。IL-1结合到IL-1的I类受体(IL-1RI)而促进形成包含IL-1RI, AcP, MyD88和IRAKs的复合体(2)。IRAK在IL-1刺激后能够在短时间内自磷酸化。接着IRAK从IL-1RI复合体上解离，泛素化并与两个膜结合蛋白 TAB2 和TRAF6结合。IRAK-TRAF6-TAB2复合体释放到细胞质中并激活蛋白激酶的级联反应，包括TAK1、IKKs 和应激活化激酶 (3)。与IL-1R/TLR (Toll样受体)连接后, IRAK1和IRAK4通过接头蛋白MyD88而迅速的招募到受体上(4)。IRAK1通过IRAK4在Thr209 和 Thr387位点磷酸化 (5), 紧接着在多个不同域自磷酸化。

1. Dinarello, C.A. (1996) Blood 87, 2095-147.
2. Takaesu, G. et al. (2001) Mol Cell Biol 21, 2475-84.
3. Janssens, S. and Beyaert, R. (2003) Mol Cell 11, 293-302.
4. Gottipati, S. et al. (2008) Cell Signal 20, 269-76.
5. Kollewe, C. et al. (2004) J Biol Chem 279, 5227-36.

原厂资料：

Specificity / Sensitivity

IRAK4 Antibody detects endogenous levels of total IRAK4. Cross-reactivity was not detected with other family members.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding lysine 41 of human IRAK4. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Interleukin-1 (IL-1) receptor-associated kinase (IRAK) is a serine/threonine-specific kinase that can be coprecipitated in an IL-1-inducible manner with the IL-1 receptor (1). The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2, IRAK3/IRAK-M and IRAK4). The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1RI, AcP, MyD88 and IRAKs (2). IRAK undergoes autophosphorylation shortly after IL-1 stimulation. The subsequent events involve IRAK dissociation from the IL-1RI complex, its ubiquitination and its association with two membrane-bound proteins: TAB2 and TRAF6. The resulting IRAK-TRAF6-TAB2 complex is then released into the cytoplasm and activates protein kinase cascades, which include TAK1, IKKs and the stress-activated kinases (3).Upon IL-1R/TLR (Toll-Like Receptor) ligation, IRAK1 and IRAK4 are rapidly recruited to the receptor by the adaptor MyD88 (4). IRAK1 is phosphorylated by IRAK4 at Thr209 and Thr387 (5), followed by sequential autohyperphosphorylation in various domains.

1. Dinarello, C.A. (1996) Blood 87, 2095-147.
2. Takaesu, G. et al. (2001) Mol Cell Biol 21, 2475-84.
3. Janssens, S. and Beyaert, R. (2003) Mol Cell 11, 293-302.
4. Gottipati, S. et al. (2008) Cell Signal 20, 269-76.
5. Kollewe, C. et al. (2004) J Biol Chem 279, 5227-36.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody