NG2 Antibody

• 产品编号：CST-4235S      品牌：CST       原厂货号：4235S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

NG2 Antibody 兔多抗识别内源性的总NG2蛋白。该多克隆抗体由合成的人类NG2蛋白His2260附近肽段免疫动物而制成。该抗体使用多肽亲和层析纯化而得。硫酸软骨素蛋白多糖NG2是I型膜蛋白，在包括少突胶质前体细胞和多种肿瘤细胞等的神经胶质细胞亚群表达。正常前体细胞和肿瘤细胞会迁移和增殖，但有证据表明，这些细胞可能无法在同一时间进行两项活动。然而，NG2的参与，促进细胞增殖和活动（1）。细胞外区域的NG2吸收了多余的生长因子，并结合生长因子受体和细胞外基质配体，如纤维连接蛋白，胶原蛋白和层粘连蛋白。细胞质区域被RAC，Cdc42和P130 CAS激活（2）。PKCα磷酸化NG2的Thr2256，触发NG2从apical microprocesses到板状伪足的分布，并伴随细胞活力的增强（3）。ERK磷酸化NG2的Thr2314，刺激细胞的增殖（4）。

1. Karram, K. et al. (2005) J Anat 207, 735-44.
2. Eisenmann, K.M. et al. (1999) Nat Cell Biol 1, 507-13.
3. Makagiansar, I.T. et al. (2004) J Biol Chem 279, 55262-70.
4. Makagiansar, I.T. et al. (2007) J Cell Biol 178, 155-65.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse

Specificity / Sensitivity

NG2 Antibody detects endogenous levels of total NG2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding His2260 of human NG2. Antibodies are purified by peptide affinity chromatography.

Background

The chondroitin sulfate proteoglycan NG2 is a type I membrane protein expressed by subpopulations of glia including oligodendroglial precursor cells and a variety of tumor cells. Normal precursor cells and malignant tumor cells migrate and proliferate, but there is evidence that cells may not be able to engage in both activities at the same time. However, NG2 is involved in promoting both proliferation and motility (1). The extracellular domain of NG2 sequesters growth factors and binds to both growth factor receptors and extracellular matrix ligands such as fibronectin, collagens and laminin. The cytoplasmic domain is involved in activating Rac, Cdc42 and p130 Cas (2). PKCα phosphorylates NG2 at Thr2256, triggering the redistribution of NG2 from apical microprocesses to lamellipodia accompanied by enhanced cell motility (3). ERK phosphorylates NG2 at Thr2314, stimulating cell proliferation (4).

1. Karram, K. et al. (2005) J Anat 207, 735-44.
2. Eisenmann, K.M. et al. (1999) Nat Cell Biol 1, 507-13.
3. Makagiansar, I.T. et al. (2004) J Biol Chem 279, 55262-70.
4. Makagiansar, I.T. et al. (2007) J Cell Biol 178, 155-65.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody