Phospho-RCC1 (Ser11) Antibody

• 产品编号：CST-4210S      品牌：CST       原厂货号：4210S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-RCC1 (Ser11) Antibody检测仅在Ser11位点磷酸化的内源性RCC1总蛋白。通过人工合成人源RCC1蛋白Ser11位点相应的磷酸化片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。Ras家族小GTPase Ran涉及细胞核膜的形成、有丝分裂纺锤体的装配和细胞核运输 (1,2)。像其它小GTPases一样，Ran蛋白以它的GTP结合形式是活性的而以它的GDP结合形式是非活性的。通过鸟嘌呤核苷酸交换因子(GEF)活性的细胞核定位，细胞核RanGTP浓度被维持，GEF催化GDP交换成GTP。Regulator of chromatin condensation 1 (RCC1)是已知的RanGEF(3)。RCC1蛋白是贯穿细胞周期的动态染色质结合形式，并且这个定位对于有丝分裂的正常进行是需要的(4,5)。RCC1蛋白与染色质的合适关联通过氨基端磷酸化(5,6) 和甲基化(7)被调节。在组蛋白修饰下，RCC1蛋白调节RanGTP水平，在凋亡期间这些调节细胞核输入(8)。在有丝分裂期间，很可能通过cyclin B/cdc2使RCC1蛋白在Ser11位点磷酸化(9-11)。该位点的磷酸化可能在RCC1蛋白与染色质相互作用和RCC1 RanGEF活性中起到重要作用(6)。

1. Quimby, B.B. and Dasso, M. (2003) Curr Opin Cell Biol 15, 338-44.
2. Hetzer, M. et al. (2002) Nat Cell Biol 4, E177-84.
3. Moore, W. et al. (2002) Curr Biol 12, 1442-7.
4. Ohtsubo, M. et al. (1989) J Cell Biol 109, 1389-97.
5. Li, H.Y. and Zheng, Y. (2004) Genes Dev 18, 512-27.
6. Hutchins, J.R. et al. (2004) Curr Biol 14, 1099-104.
7. Chen, T. et al. (2007) Nat Cell Biol 9, 596-603.
8. Wong, C.H. et al. (2009) Nat Cell Biol 11, 36-45.
9. Horiike, Y. et al. (2009) Mol Biol Rep 36, 717-23.
10. Dephoure, N. et al. (2008) Proc Natl Acad Sci U S A 105, 10762-7.
11. Hood, F.E. and Clarke, P.R. (2007) J Cell Sci 120, 3436-45.

原厂资料：

Specificity / Sensitivity

Phospho-RCC1 (Ser11) Antibody recognizes endogenous levels of RCC1 protein only when phosphorylated at Ser11.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser11 of human RCC1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The Ras family small GTPase Ran is involved in nuclear envelope formation, assembly of the mitotic spindle and nuclear transport (1,2). Like other small GTPases, Ran is active in its GTP-bound form and inactive in its GDP-bound form. Nuclear RanGTP concentration is maintained through nuclear localization of guanine nucleotide exchange factor (GEF) activity, which catalyzes the exchange of bound GDP for GTP. RCC1 (regulator of chromatin condensation 1) is the only known RanGEF (3). RCC1 is dynamically chromatin-bound throughout the cell cycle, and this localization is required for mitosis to proceed normally (4,5). Appropriate association of RCC1 with chromatin is regulated through amino-terminal phosphorylation (5,6) and methylation (7). RCC1 regulation of RanGTP levels in response to histone modifications regulates nuclear import during apoptosis (8). In mitosis RCC1 is phosphorylated at Ser11, possibly by cyclinB/cdc2 (9-11). This phosphorylation may play a role in RCC1 interaction with chromatin and its RanGEF activity (6).

1. Quimby, B.B. and Dasso, M. (2003) Curr Opin Cell Biol 15, 338-44.
2. Hetzer, M. et al. (2002) Nat Cell Biol 4, E177-84.
3. Moore, W. et al. (2002) Curr Biol 12, 1442-7.
4. Ohtsubo, M. et al. (1989) J Cell Biol 109, 1389-97.
5. Li, H.Y. and Zheng, Y. (2004) Genes Dev 18, 512-27.
6. Hutchins, J.R. et al. (2004) Curr Biol 14, 1099-104.
7. Chen, T. et al. (2007) Nat Cell Biol 9, 596-603.
8. Wong, C.H. et al. (2009) Nat Cell Biol 11, 36-45.
9. Horiike, Y. et al. (2009) Mol Biol Rep 36, 717-23.
10. Dephoure, N. et al. (2008) Proc Natl Acad Sci U S A 105, 10762-7.
11. Hood, F.E. and Clarke, P.R. (2007) J Cell Sci 120, 3436-45.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.