Phospho-MKK7 (Ser271/Thr275) Antibody

• 产品编号：CST-4171S      品牌：CST       原厂货号：4171S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-MKK7 (Ser171/Thr275) Antibody兔多抗能检测内源性Ser271位点和Thr275位点磷酸化的MKK7蛋白水平。该抗体不能与磷酸化的SEK1/MKK4、其他磷酸化的MAPKK发生交叉反应。该多克隆抗体能够采用合成的与人源MKK7蛋白Ser271/Thr275位点周围残基相一致的磷酸化肽段免疫动物而获得。MKK7是一种MAPKK，它是JNK/SAPK通路的特异性活化因子(1,2)。MKK7能被TNFα以及其他环境压力显著激活，然而能激活 p38、JNK/SAPK通路的SEK1/MKK4却不能被TNFα所激活(2)。对MAPKK家族成员活化环的序列比对表明，MKK7蛋白的Ser271和Thr275位点是潜在的磷酸化位点，这两个位点对激酶的活性十分重要。

1. Tournier, C. et al. (1999) Mol. Cell. Biol. 19, 1569-1581.
2. Moriguchi, T. et al. (1997) EMBO J. 16, 7045-7053.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

Phospho-MKK7 (Ser171/Thr275) Antibody detects endogenous levels of MKK7 phosphorylated at serine 271 and threonine 275. This antibody does not cross-react with phosphorylated SEK1/MKK4 or other phosphorylated MAP kinase kinases.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser271/Thr275 of human MKK7. Antibodies are purified by protein A and peptide affinity chromatography.

Background

MKK7 is a MAP kinase kinase that serves as a specific activator of JNK/SAPK pathway (1,2). MKK7 is activated strongly by TNFα, as well as other environmental stresses, whereas SEK1/MKK4, which activates both p38 and JNK/SAPK pathways, is not activated by TNFα (2). Sequence alignment of the activation loop of the MAP kinase kinase family members indicates that Ser271 and Thr275 are potential phosphorylation sites that are crucial for the kinase acivity.

1. Tournier, C. et al. (1999) Mol. Cell. Biol. 19, 1569-1581.
2. Moriguchi, T. et al. (1997) EMBO J. 16, 7045-7053.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody