Phospho-HSL (Ser565) Antibody

• 产品编号：CST-4137S      品牌：CST       原厂货号：4137S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-HSL (Ser565) Antibody用于检测内源性的在Ser565被AMPK磷酸化的HSL蛋白水平，该抗体不与Ser563磷酸化的HSL蛋白交叉反应。多克隆抗体是通过合成对应于人HSL蛋白Ser554（大鼠HSL的Ser565）序列的肽段免疫动物获得，抗体经Protein A和肽亲和层析纯化制得。激素敏感型脂肪酶（HSL）催化甘油三酯的水解反应，这是脂肪分解过程的限速步骤。脂分解性的刺激因素会激活腺苷酸环化酶，因而增加了胞内cAMP的水平 ，进一步会增加蛋白激酶A的活性。PKA会磷酸化HSL的 Ser563, Ser659 和Ser660，磷酸化会激活HSL（1,2）。相反，AMPK可以磷酸化HSL的Ser565，这会阻 止HSL被PKA对其Ser563磷酸化，这会抑制HSL的活性（2,3）。最近的研究表明，p44/42 MAPKs 对HSL　Ser600的磷酸化也会增加它的活性（4）。

1. Degerman, E. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 533-537.
2. Anthonsen, M.W. et al. (1998) J. Biol. Chem. 273, 215-221.
3. Garton, A.J. and Yeaman, S.J. (1990) Eur. J. Biochem. 191, 245-250.
4. Greenberg, A.S. et al. (2001) J. Biol. Chem. 276, 45456-45461.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Human, Rat

Specificity / Sensitivity

Phospho-HSL (Ser565) Antibody detects endogenous levels of HSL only when phosphorylated at Ser565 by AMPK. This antibody does not cross-react with Ser563 phosphorylated HSL.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser554 of human HSL (Ser565 of rat HSL). Antibodies are purified by protein A and peptide affinity chromatography.

Background

HSL (hormone-sensitive lipase) catalyzes the hydrolysis of triacylglycerol, the rate-limiting step in lipolysis. Lipolytic stimuli activate adenylyl cyclase and thus increase intracellular cAMP levels, which in turn activate protein kinase A (PKA). PKA phosphorylates HSL at Ser563, Ser659 and Ser660, which stimulates HSL activity (1,2). In contrast, AMPK phosphorylates HSL at Ser565, which reduces HSL phosphorylation at Ser563 by PKA and inhibits HSL activity (2,3). Recent work indicates that phosphorylation at Ser600 by p44/42 MAPKs also enhances the enzymatic activity of HSL (4).

1. Degerman, E. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 533-537.
2. Anthonsen, M.W. et al. (1998) J. Biol. Chem. 273, 215-221.
3. Garton, A.J. and Yeaman, S.J. (1990) Eur. J. Biochem. 191, 245-250.
4. Greenberg, A.S. et al. (2001) J. Biol. Chem. 276, 45456-45461.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody