TLK1 Antibody

• 产品编号：CST-4125S      品牌：CST       原厂货号：4125S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

TLK1 Antibody 能够检测内有效TLK1总蛋白。该抗体可能与TLK2发生交叉反应。该多克隆抗体是由合成的人源的针对TLK1蛋白丝氨酸（183位）的肽段免疫动物，采用蛋白A和多肽亲和层析技术纯化生产的。Tousled样激酶(TLK1 and TLK2)属于核丝氨酸/苏氨酸激酶，因其与来自拟南芥的Tousled基因的同源性而得名，对于花的发育必不可少(1)。TLKs的激酶活性是细胞周期调控的，在S期活性最高(1)。TLK似乎在染色质装配和DNA损伤检验点调控中起重要作用(1,2)。TLK1对于线虫胚胎发育过程中的适当转录是必需的(3)。TLK的底物包括人类染色质装配因子Asf，它在DNA复制和修复偶联染色质装配过程中起作用(2)。S期DNA损伤时，TLK被最大程度的活化，导致TLK活性抑制(1)。这种抑制需要ATM和Chk1(4,5)。S期DNA损伤激活ATM和相关激酶ATR，使Chk1/Chk2磷酸化，阻遏有丝分裂期的转换(6)。Chk1体内/外都可以磷酸化TLK1的695位丝氨酸，引起TLK1的活性抑制(4)。该过程很可能提供了一种在DNA损伤事件中减慢TLK控制的染色质装配过程。

1. Sillje, H. H. et al. (1999) EMBO J. 18, 5691-5702.
2. Sillje, H.H. and Nigg, E.A. (2001) Curr. Biol. 11, 1068-1073.
3. Han, Z. et al. (2003) Curr. Biol. 13, 1921-1929.
4. Groth, A. et al. (2003) EMBO J. 22, 1676-1687.
5. Krause, D. R. et al. (2003) Oncogene 22, 5927-5937.
6. Kastan, M.B. and Lim, D.S. (2000) Nat. Rev. Mol. Cell Biol. 1, 179-186.

原厂资料：

Specificity / Sensitivity

TLK1 Antibody detects endogenous levels of total TLK1.The antibody may cross-react with TLK2.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues adjacent to Ser183 of human TLK1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Tousled-like kinases (TLK1 and TLK2) are nuclear serine/threonine kinases named for their homology to the Tousled gene from Arabidopsis thaliana, essential for flower development (1). The kinase activities of the TLKs are cell cycle regulated, with maximal activity during S phase (1). TLK appears to play a role in chromatin assembly and DNA damage checkpoint regulation (1,2). In C. elegans, TLK1 is essential for appropriate transcription during embryonic development (3). Substrates for TLK include the human chromatin assembly factor Asf, which functions in DNA replication- and repair-coupled chromatin assembly (2). DNA damage during S phase, when TLK is maximally active, leads to inhibition of TLK activity (1). This inhibition requires ataxia mutated kinase (ATM) and Chk1 (4,5). ATM and the related kinase ATR are activited by DNA damage during S phase, phosphorylate Chk1/Chk2, and block the transition into mitosis (6). Chk1 phosphorylates TLK1 on Ser695 in vitro and in vivo, leading to inhibition of TLK1 activity (4). This process likely provides a mechanism to slow the chromatin assembly processes controlled by TLK in the event of DNA damage.

1. Sillje, H. H. et al. (1999) EMBO J. 18, 5691-5702.
2. Sillje, H.H. and Nigg, E.A. (2001) Curr. Biol. 11, 1068-1073.
3. Han, Z. et al. (2003) Curr. Biol. 13, 1921-1929.
4. Groth, A. et al. (2003) EMBO J. 22, 1676-1687.
5. Krause, D. R. et al. (2003) Oncogene 22, 5927-5937.
6. Kastan, M.B. and Lim, D.S. (2000) Nat. Rev. Mol. Cell Biol. 1, 179-186.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.