HSL Antibody

• 产品编号：CST-4107S      品牌：CST       原厂货号：4107S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

HSL Antibody用于检测内源性HSL总蛋白水平。多克隆抗体通过用对应于人源HSL蛋白序列的合成肽免疫动物制备而得，抗体经蛋白A和肽段亲和层析纯化制得。激素敏感型脂肪酶（HSL）催化甘油三酯的水解反应，这是脂肪分解过程的限速步。脂分解性的刺激因素会激活腺苷酸环化酶，因而增加了胞内cAMP的水平，进一步会增加蛋白激酶A的活性。 PKA会磷酸化HSL的 Ser563, Ser659 和Ser660，磷酸化会激活HSL（1,2）。相反的，AMPK可以磷酸化HSL的Ser565，这会阻止HSL被PKA对其Ser563磷酸化，这会抑制HSL的活性（2,3）。最近的研 究表明，p44/42 MAPKs 对HSL　Ser600的磷酸化也会增加它的活性（4）。

1. Degerman, E. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 533-537.
2. Anthonsen, M.W. et al. (1998) J. Biol. Chem. 273, 215-221.
3. Garton, A.J. and Yeaman, S.J. (1990) Eur. J. Biochem. 191, 245-250.
4. Greenberg, A.S. et al. (2001) J. Biol. Chem. 276, 45456-45461.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Human

Specificity / Sensitivity

HSL Antibody detects endogenous levels of total HSL protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human HSL. Antibodies are purified by peptide affinity chromatography.

Background

HSL (hormone-sensitive lipase) catalyzes the hydrolysis of triacylglycerol, the rate-limiting step in lipolysis. Lipolytic stimuli activate adenylyl cyclase and thus increase intracellular cAMP levels, which in turn activate protein kinase A (PKA). PKA phosphorylates HSL at Ser563, Ser659 and Ser660, which stimulates HSL activity (1,2). In contrast, AMPK phosphorylates HSL at Ser565, which reduces HSL phosphorylation at Ser563 by PKA and inhibits HSL activity (2,3). Recent work indicates that phosphorylation at Ser600 by p44/42 MAPKs also enhances the enzymatic activity of HSL (4).

1. Degerman, E. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 533-537.
2. Anthonsen, M.W. et al. (1998) J. Biol. Chem. 273, 215-221.
3. Garton, A.J. and Yeaman, S.J. (1990) Eur. J. Biochem. 191, 245-250.
4. Greenberg, A.S. et al. (2001) J. Biol. Chem. 276, 45456-45461.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody