GAD2 Antibody

• 产品编号：CST-3988S      品牌：CST       原厂货号：3988S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

GAD2 Antibody 兔多抗识别内源性的总GAD2蛋白。该多克隆抗体由合成的人类GAD2蛋白Ala150附近肽段免疫动物而制成。该抗体使用多肽亲和层析纯化而得。谷氨酸脱羧酶（GAD）负责从L-谷氨酸合成重要的神经递质γ-氨基丁酸（GABA）（1）。GAD1（GAD67）和GAD2（GAD65）在神经系统和内分泌系统表达（2），被认为分别参与突触传递（3）和胰岛素分泌（4）。GAD2的自身抗体可作为I型糖尿病的生物标记之一（5）。许多患成年期发病的僵人综合症（SPS）的患者也表达抗体GAD2（6）。

1. Kaufman, D.L. et al. (1991) J Neurochem 56, 720-3.
2. Feldblum, S. et al. (1993) J Neurosci Res 34, 689-706.
3. Gao, B. and Moore, R.Y. (1996) J Biol Rhythms 11, 172-9.
4. Rubi, B. et al. (2001) J Biol Chem 276, 36391-6.
5. Gilliam, L.K. et al. (2004) Clin Exp Immunol 138, 337-41.
6. Skorstad, G. et al. (2008) Eur J Neurol 15, 973-80.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Human

Specificity / Sensitivity

GAD2 Antibody detects endogenous levels of total GAD2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Ala150 of human GAD2. Antibodies are purified by peptide affinity chromatography.

Background

The enzyme glutamate decarboxylase (GAD) is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid (1). GAD1 (GAD67) and GAD2 (GAD65) are expressed in nervous and endocrine systems (2) and are thought to be involved in synaptic transmission (3) and insulin secretion (4), respectively. Autoantibodies against GAD2 may serve as markers for type I diabetes (5). Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2 (6).

1. Kaufman, D.L. et al. (1991) J Neurochem 56, 720-3.
2. Feldblum, S. et al. (1993) J Neurosci Res 34, 689-706.
3. Gao, B. and Moore, R.Y. (1996) J Biol Rhythms 11, 172-9.
4. Rubi, B. et al. (2001) J Biol Chem 276, 36391-6.
5. Gilliam, L.K. et al. (2004) Clin Exp Immunol 138, 337-41.
6. Skorstad, G. et al. (2008) Eur J Neurol 15, 973-80.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.