EphB1 (5F10) Mouse mAb

• 产品编号：CST-3980S      品牌：CST       原厂货号：3980S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

磷酸化EphB1的小鼠源单克隆抗体(5F10)能检测到内源的EphB1蛋白的表达。本抗体不与家族其它成员发生交叉反应。单克隆抗体通过用多肽免疫动物得到，该多肽是根据人的EphB1蛋白19-133位的氨基酸序列合成的。Eph受体是研究的最为清楚的酪氨酸激酶受体家族(RTKs)。根据其序列的相似性和一子集配体的倾向性可将它们分为两组：结合基磷脂酰肌醇锚定的麻黄素A配体的EphA受体； 结合具有跨膜区和胞内区的麻黄素B蛋白的EphB 受体(1,2)。Eph 受体及其配体与多种疾病相关，包括肿瘤（3）。Eph A 和B配体都有双重功能。作为RTK配体，麻黄素激活Eph受体的激酶活性以及激活表达该受体细胞的信号通路。麻黄素的胞外区的聚集足以完成这项功能（4）。麻黄素的第二项功能称为“反向信号调节”，凭借胞内区的酪氨酸磷酸化，能够与表达配体的细胞中许多激活信号通路的其它蛋白相互作用（5）。许多刺激都能使麻黄素B的酪氨酸位点磷酸化，包括与麻黄素B受体结合、Src激酶的激活以及受PDGF和 FGF刺激 (6)。酪氨酸 324/327的磷酸化被认为是体内麻黄素 B1的主要磷酸化位点（7）。EphB1是Eph酪氨酸激酶受体家族的成员之一，它在神经系统发育、血管生成以及神经突触形成和成熟等多种不同的生物过程中起重要作用（8）。在一些肿瘤组织中有某些Eph受体的高表达或低表达。EphB1被报道参与结直肠癌的发生（9）。

1. Wilkinson, D.G. (2000) Int Rev Cytol 196, 177-244.
2. Klein, R. (2001) Curr Opin Cell Biol 13, 196-203.
3. Dodelet, V.C. and Pasquale, E.B. (2000) Oncogene 19, 5614-9.
4. Holder, N. and Klein, R. (1999) Development 126, 2033-44.
5. Brückner, K. et al. (1997) Science 275, 1640-3.
6. Palmer, A. et al. (2002) Mol Cell 9, 725-37.
7. Kalo, M.S. et al. (2001) J Biol Chem 276, 38940-8.
8. Fasen, K. et al. (2008) Traffic 9, 251-266.
9. Sheng, Z. et al. (2008) Pathobiology 75, 274-280.

原厂资料：

Specificity / Sensitivity

EphB1 (5F10) Mouse mAb detects endogenous levels of EphB1 protein. It does not cross-react with other family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide. corresponding to residues surrounding amino acids 19-133 of human EphB1. 

Background

The Eph receptors are the largest known family of receptor tyrosine kinases (RTKs). They can be divided into two groups based on sequence similarity and on their preference for a subset of ligands: EphA receptors bind to a glycosylphosphatidylinositol-anchored ephrin A ligand; EphB receptors bind to ephrin B proteins that have a transmembrane and cytoplasmic domain (1,2). Eph receptors and ligands may be involved in many diseases including cancer (3). Both ephrin A and B ligands have dual functions. As RTK ligands, the ephrins stimulate the kinase activity of the Eph receptors and activate signaling pathways in receptor-expressing cells. The ephrin extracellular domain is sufficient for this function as long as it is clustered (4). The second function of ephrins has been described as "reverse signaling", whereby the cytoplasmic domain becomes tyrosine phosphorylated, allowing interactions with other proteins that may activate signaling pathways in the ligand-expressing cells (5). Various stimuli can induce tyrosine phosphorylation of ephrin B, including binding to EphB receptors, activation of Src kinase, and stimulation by PDGF and FGF (6). Tyrosines 324/327 have been identified as major phosphorylation sites of ephrin B1 in vivo (7).

EphB1 is a member of the Eph family of receptor tyrosine kinases that plays an important role in diverse biological processes including nervous system development, angiogenesis, and neural synapsis formation and maturation (8). Over- or underexpression of certain Eph receptors has been found in some cancer tissues. EphB1 has been shown to be involved in the tumorigenesis of colorectal cancer (9).

1. Wilkinson, D.G. (2000) Int Rev Cytol 196, 177-244.
2. Klein, R. (2001) Curr Opin Cell Biol 13, 196-203.
3. Dodelet, V.C. and Pasquale, E.B. (2000) Oncogene 19, 5614-9.
4. Holder, N. and Klein, R. (1999) Development 126, 2033-44.
5. Brückner, K. et al. (1997) Science 275, 1640-3.
6. Palmer, A. et al. (2002) Mol Cell 9, 725-37.
7. Kalo, M.S. et al. (2001) J Biol Chem 276, 38940-8.
8. Fasen, K. et al. (2008) Traffic 9, 251-266.
9. Sheng, Z. et al. (2008) Pathobiology 75, 274-280.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.