Gα(o) Antibody

• 产品编号：CST-3975S      品牌：CST       原厂货号：3975S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Gα(o)抗体识别内源性的全部Gα(o)蛋白。该多克隆抗体用与人类Gα(o)中Arg15位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。异三聚鸟嘌呤核苷酸结合蛋白（G蛋白）由α、β和γ亚基构成，介导激素、神经递质、趋化因子和感觉刺激的作用。到今天为止，根据结构和功能的相似性，超过20个亚基分为四个家族，分别是Gα(s), Gα(i/o), Gα(q)和Gα(12)（1,2）。Gα(q)/Gα(11)的Tyr356磷酸化对G蛋白激活是至关重要的，因为苯丙氨酸替代Tyr356改变了Gα与受体的相互作用，并消除了配体诱导的IP3的形成（3）。Gα(o)是脑内最丰富的G蛋白，与五羟色胺、多巴胺、GABA(B)、阿片、谷氨酸和胆碱能受体相偶联（4）。Gα(o)-/-小鼠会发生震颤、癫痫和运动协调障碍等神经缺陷（5）。

1. Offermanns, S. (2001) Oncogene 20, 1635-42.
2. Pierce, K.L. et al. (2002) Nat Rev Mol Cell Biol 3, 639-50.
3. Umemori, H. et al. (1997) Science 276, 1878-81.
4. Jiang, M. and Bajpayee, N.S. (2009) Neurosignals 17, 23-41.
5. Jiang, M. et al. (1998) Proc Natl Acad Sci USA 95, 3269-74.

原厂资料：

Specificity / Sensitivity

Gα(o) Antibody detects endogenous levels of total Gα(o) protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg15 of human Gα(o). Antibodies are purified by protein A and peptide affinity chromatography.

Background

Heterotrimeric guanine nucleotide-binding proteins (G proteins) consist of α, β and γ subunits and mediate the effects of hormones, neurotransmitters, chemokines and sensory stimuli. To date, over 20 known Gα subunits have been classified into four families, Gα(s), Gα(i/o), Gα(q) and Gα(12), based on structural and functional similarities (1,2). Phosphorylation of Tyr356 of Gα(q)/Gα(11) is essential for activation of the G protein, since phenylalanine substitution for Tyr356 changes the interaction of Gα with receptors and abolishes ligand-induced IP3 formation (3).Gα(o) is the most abundant G protein in the brain and couples to serotonin, dopamine, GABA (B), opioid, glutamate and cholinergic receptors (4). Gα(o) -/- mice have neurological defects such as tremors, seizures and poor motor coordination (5).

1. Offermanns, S. (2001) Oncogene 20, 1635-42.
2. Pierce, K.L. et al. (2002) Nat Rev Mol Cell Biol 3, 639-50.
3. Umemori, H. et al. (1997) Science 276, 1878-81.
4. Jiang, M. and Bajpayee, N.S. (2009) Neurosignals 17, 23-41.
5. Jiang, M. et al. (1998) Proc Natl Acad Sci USA 95, 3269-74.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody