GRB2 Antibody

• 产品编号：CST-3972S      品牌：CST       原厂货号：3972S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

GRB2 Antibody兔多抗能检测内源性GRB2总蛋白水平。该多克隆抗体是采用合成的与人源GRB2蛋白羧端残基相一致的合成肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。生长因子受体结合蛋白2 (GRB2)是一种接头蛋白，与RTK信号转导相关。GRB2蛋白的SH2结构域能够结合酪氨酸磷酸化的蛋白，如 EGFR, IRS-1, Shc 和 Gab1 (1)。GRB2蛋白的SH3结构域能够与Sos相互结合，进而提高Ras蛋白的GTP结合活性，导致MAPK和其他信号转导途径的活化。GRB2中的Tyr209被 Bcr-Abl和EGFR磷酸化后，能够使其与Sos解离，从而对下游通路形成负调控。 (2)

1. Pawson, T. and Scott, J.D. (1997) Science 278, 2075-80.
2. Li, S. et al. (2001) EMBO J 20, 6793-804.

原厂资料：

Specificity / Sensitivity

GRB2 Antibody detects endogenous levels of total GRB2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues of human GRB2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Growth factor receptor-binding protein 2 (GRB2) is a adaptor protein that is involved in RTK signal transduction. The SH2 domain of GRB2 binds to tyrosine phosphorylated proteins such as EGFR, IRS-1, Shc and Gab1 (1). The SH3 domain of GRB2 associates with Sos, which stimulates the GTP binding activity of Ras, leading to the activation of the MAP kinase and other signaling pathways. Phosphorylation of tyrosine 209 of GRB2 by Bcr-Abl and EGFR abolishes its association with Sos and negatively regulates downstream signaling (2).

1. Pawson, T. and Scott, J.D. (1997) Science 278, 2075-80.
2. Li, S. et al. (2001) EMBO J 20, 6793-804.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.