Nur77 (D63C5) XP? Rabbit mAb

• 产品编号：CST-3960P      品牌：Cell Signaling Technology       原厂货号：3960P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Nur77 (D63C5) XP® Rabbit mAb能够检测内源性水平总的人Nur77蛋白。该单克隆抗体通过用合成肽免疫动物制备，该合成肽是人Nur77蛋白亮氨酸（535位）附近的残基。.Nur77也称为TR3和NGFI-B，是一种即早反应基因和类固醇/甲状腺/类视黄醇受体超家族的孤儿成员（1-3）。 Nur77由一个氨基末端反式激活域，一个中央DNA结合结构域和一个羧基末端配体结合域组成。Nur77的表达由多种刺激快速诱导，包括细胞凋亡、有丝分裂和应激信号（1-6）。已经提出Nur77有多个与细胞增殖、分化和细胞凋亡相关的功能。在T细胞中，Nur77已被广泛研究，它与负选择过程和TCR介导的细胞凋亡有关（5,6）。Nur77结合到特定的DNA分子，导致靶基因的调控（7）。作为一种调控细胞凋亡的可能机制，Nur77可以诱导凋亡基因的表达，如FasL和TRAIL（8,9）。 Nur77很大程度上是由多种激酶磷酸化，从而可能影响其转录活性以及其亚细胞定位（4,10,11）。Nur77从细胞核易位到线粒体，可以调节其与Bcl-2的联合，并控制细胞色素c的释放，从而引发细胞凋亡（12,13）。

1. Hazel, T.G. et al. (1988) Proc. Natl. Acad. Sci. USA 85, 8444-8448.
2. Chang, C. and Kokontis, J. (1988) Biochem. Biophys. Res. Commun. 155, 971-977.
3. Milbrandt, J. (1988) Neuron 1, 183-188.
4. Fahrner, T.J. et al. (1990) Mol. Cell. Biol. 10, 6454-6459.
5. Liu, Z.G. et al. (1994) Nature 367, 281-284.
6. Woronicz, J.D. et al. (1994) Nature 367, 277-281.
7. Wilson, T.E. et al. (1991) Science 252, 1296-1300.
8. Weih, F. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 5533-5538.
9. Rajpal, A. et al. (2003) EMBO J. 22, 6526-6536.
10. Hirata, Y. et al. (1993) J. Biol. Chem. 268, 24808-24812.
11. Hazel, T.G. et al. (1991) Mol. Cell. Biol. 11, 3239-3246.
12. Li, H. et al. (2000) Science 289, 1159-1164.
13. Lin, B. et al. (2004) Cell 116, 527-540.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

Nur77 (D63C5) XP® Rabbit mAb detects endogenous levels of total human Nur77 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu535 of human Nur77.

Background

Nur77, also known as TR3 and NGFI-B, is an immediate-early response gene and an orphan member of the steroid/thyroid/retinoid receptor superfamily (1-3). Nur77 is composed of an amino-terminal transactivation domain, a central DNA-binding domain and a carboxy-terminal ligand-binding domain. Expression of Nur77 is rapidly induced by a variety of stimuli, including apoptotic, mitogenic and stress signals (1-6). It has been proposed to have many functions related to cell proliferation, differentiation and apoptosis. Nur77 has been extensively studied in T cells where it has been implicated in the process of negative selection and TCR-mediated apoptosis (5,6). Nur77 binds to specific DNA elements leading to the regulation of target genes (7). As a possible mechanism for regulating apoptosis, Nur77 can induce the expression of apoptotic genes such as FasL and TRAIL (8,9). Nur77 is heavily phosphorylated by multiple kinases, which may affect its transactivation activity as well as its subcellular localization (4,10,11). Translocation of Nur77 from the nucleus to the mitochondria can regulate its association with Bcl-2 and control the release of cytochrome c, thereby triggering apoptosis (12,13).

1. Hazel, T.G. et al. (1988) Proc. Natl. Acad. Sci. USA 85, 8444-8448.
2. Chang, C. and Kokontis, J. (1988) Biochem. Biophys. Res. Commun. 155, 971-977.
3. Milbrandt, J. (1988) Neuron 1, 183-188.
4. Fahrner, T.J. et al. (1990) Mol. Cell. Biol. 10, 6454-6459.
5. Liu, Z.G. et al. (1994) Nature 367, 281-284.
6. Woronicz, J.D. et al. (1994) Nature 367, 277-281.
7. Wilson, T.E. et al. (1991) Science 252, 1296-1300.
8. Weih, F. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 5533-5538.
9. Rajpal, A. et al. (2003) EMBO J. 22, 6526-6536.
10. Hirata, Y. et al. (1993) J. Biol. Chem. 268, 24808-24812.
11. Hazel, T.G. et al. (1991) Mol. Cell. Biol. 11, 3239-3246.
12. Li, H. et al. (2000) Science 289, 1159-1164.
13. Lin, B. et al. (2004) Cell 116, 527-540.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.