Myc-Tag (71D10) Rabbit mAb (Biotinylated)

• 产品编号：CST-3946S      品牌：CST       原厂货号：3946S
• 产品分类：抗体 > 一抗 > 表位标签和报告基因特异性抗体
• 应用分类：

描述：

Myc-Tag (71D10) Rabbit mAb可以检测细胞中包含Myc标为标签的蛋白，此抗体可以识别与目的蛋白氨基末端或羧基末端的融合的Myc-Tag。多克隆抗体是采用与人类c-Myc (EQKLISEEDL)的410-419残基相对应的合成肽段免疫动物而生产的。此种抗体结合了生物素，已经过内部测试，可以直接在转染Myc-标签蛋白的COS细胞。在免疫印迹、免疫沉淀和免疫染色等技术中，表位标签用于标记和检测蛋白。由于它们的体积很小，它们一般不会影响标记蛋白的生化特性。Myc标为标签在细菌、酵母和哺乳动物细胞系统中广泛应用于检测重组蛋白的表达（1）。

1. Munro, S. and Pelham, H.R. (1984) EMBO J 3, 3087-93.

原厂资料：

Specificity / Sensitivity

Myc-Tag (71D10) Rabbit mAb detects recombinant proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).

Description

This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions and tested using COS cells transfected with Myc tagged protein.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1).

1. Munro, S. and Pelham, H.R. (1984) EMBO J 3, 3087-93.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM
sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, 50% glycerol.
Store at -20ºC. Do not aliquot antibody.