FoxO3a (75D8) Rabbit mAb (Biotinylated)

• 产品编号：CST-3938S      品牌：CST       原厂货号：3938S
• 产品分类：抗体 > 一抗 > 表位标签和报告基因特异性抗体
• 应用分类：

描述：

FoxO3a (75D8) Rabbit mAb (Biotinylated)兔单抗能够检测内源性的FoxO3a总蛋白。抗体不和FoxO4和 FoxO1家族的其它成员发生交叉反应。该单克隆抗体是采用合成的与人源FoxO3a蛋白Glu50位点周围序列相对应的肽段免疫动物而生产的。CST公司的抗体在最优化条件下偶联到生物素。 未偶联的FoxO3a (75D8) Rabbit mAb兔单抗 #2497能够和人、小鼠和大鼠的Fox03a 蛋白反应。 CST公司认为FoxO3a (75D8) Rabbit mAb (Biotinylated)兔单抗也能够识别以上物种的Fox03a 蛋白。Forkhead转录因子家族参与横纹肌肉瘤和急性白血病的发生（1-3）。在这个家族中，三个成员（FoxO1，FoxO4和FoxO3a）与线虫的同源基因DAF-16具有序列相似度，而DAF-16介导包括IGFR1，PI3K和Akt在内的信号通路（4-6）。活化的forkhead成员作为肿瘤抑制因子，促进细胞周期阻滞和凋亡。FOXO家族任何成员的表达上调都会激活细胞周期抑制剂p27 Kip1。Forkhead转录因子也参与TGF-β介导的p21 CIP1上调，而这个上调过程可以被PI3K负调控（7）。forkhead转录因子被Akt在Thr24，Ser256以及Ser319磷酸化后而失活，导致出核转运与转录因子活性的抑制（8），最终导致增殖增加。Forkhead转录因子活性也可以被去乙酰化酶sirtuin(SirT1)抑制（9）。

1. Anderson, M.J. et al. (1998) Genomics 47, 187-99.
2. Galili, N. et al. (1993) Nat Genet 5, 230-5.
3. Borkhardt, A. et al. (1997) Oncogene 14, 195-202.
4. Nakae, J. et al. (1999) J Biol Chem 274, 15982-5.
5. Rena, G. et al. (1999) J Biol Chem 274, 17179-83.
6. Guo, S. et al. (1999) J Biol Chem 274, 17184-92.
7. Seoane, J. et al. (2004) Cell 117, 211-23.
8. Arden, K.C. (2004) Mol Cell 14, 416-8.
9. Yang, Y. et al. (2005) EMBO J 24, 1021-32.

原厂资料：

Specificity / Sensitivity

FoxO3a (75D8) Rabbit mAb (Biotinylated) detects endogenous levels of total FoxO3a protein. The antibody does not cross-react with other members of the FoxO4 or FoxO1 families.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu50 of human FoxO3a.

Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The unconjugated FoxO3a (75D8) Rabbit mAb #2497 reacts with human, mouse and rat Fox03a protein. CST expects that FoxO3a (75D8) Rabbit mAb (Biotinylated) will also recognize Fox03a in these species.

Background

The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9).

1. Anderson, M.J. et al. (1998) Genomics 47, 187-99.
2. Galili, N. et al. (1993) Nat Genet 5, 230-5.
3. Borkhardt, A. et al. (1997) Oncogene 14, 195-202.
4. Nakae, J. et al. (1999) J Biol Chem 274, 15982-5.
5. Rena, G. et al. (1999) J Biol Chem 274, 17179-83.
6. Guo, S. et al. (1999) J Biol Chem 274, 17184-92.
7. Seoane, J. et al. (2004) Cell 117, 211-23.
8. Arden, K.C. (2004) Mol Cell 14, 416-8.
9. Yang, Y. et al. (2005) EMBO J 24, 1021-32.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 2 mg/ml BSA, 50% glycerol. Store at -20ºC.
Do not aliquot antibody.