Tropomyosin-1/3 (D17B8) Rabbit mAb

• 产品编号：CST-3913S      品牌：CST       原厂货号：3913S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Tropomyosin-1/3 (D17B8) Rabbit mAb检测内源性tropomyosin-1和/或tropomyosin-3总蛋白。通过人工合成人源tropomyosin-1蛋白Leu274位点周围相应的多肽片段去免疫动物从而制备出单克隆抗体。Tropomyosin-1 (TPM1)蛋白属于tropomyosin家族的高分子成员(1,2)。该蛋白质存在一个 α-螺旋卷曲螺旋构象，并且以一种紧密方式结合多重活性单体去稳定和调节肌动蛋白纤维(3)。Tropomyosins蛋白在肌细胞和非肌性细胞履行职能。在肌细胞中，tropomyosins蛋白与troponin蛋白复合物有联系，并且在脊椎动物条纹肌肉收缩的钙离子依赖的调节中起着核心作用。在非肌性细胞中，tropomyosins涉及细胞支架的肌动蛋白纤维的形成和稳定，从而确定正常细胞内进程(1,2)。tropomysin-1蛋白的突变已经被报道作为一个扩张的心肌疾病的原因(4)。Tropomyosin-1蛋白也作为一个肿瘤抑制因子，并且许多恶性肿瘤证明tropomyosin-1蛋白表达的下调(5-8)。Tropomyosin-1蛋白通过Erk/DAPK通路使Ser283位点磷酸化，这促进了在氧化应激下应力纤维形成(9-10)。

1. Perry, S.V. (2001) J Muscle Res Cell Motil 22, 5-49.
2. Lin, J.J. et al. (2008) Adv Exp Med Biol 644, 201-22.
3. Brown, J.H. et al. (2001) Proc Natl Acad Sci USA 98, 8496-501.
4. Rajan, S. et al. (2007) Circ Res 101, 205-14.
5. Bharadwaj, S. et al. (2004) J Biol Chem 279, 14039-48.
6. Mahadev, K. et al. (2002) Exp Cell Res 279, 40-51.
7. Varga, A.E. et al. (2005) Oncogene 24, 5043-52.
8. Raval, G.N. et al. (2003) Oncogene 22, 6194-203.
9. Houle, F. et al. (2003) Mol Biol Cell 14, 1418-32.
10. Houle, F. et al. (2007) J Cell Sci 120, 3666-77.

原厂资料：

Specificity / Sensitivity

Tropomyosin-1/3 (D17B8) Rabbit mAb detects endogenous levels of total tropomyosin-1 and/or tropomyosin-3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu274 of human tropomyosin-1 protein.

Background

Tropomyosin-1 (TPM1) belongs to the high molecular weight members of tropomyosin family (1,2). The protein exists in an alpha-helical coiled-coil conformation and binds multiple acting monomers in a tight manner to stabilize and regulate the actin filament (3). Tropomyosins fullfill functions in muscle and non-muscle cells. In muscle cells, tropomyosins associate with the troponin complex and play a central role in the calcium-dependent regulation of striated muscle contraction in vertebrates. In non-muscle cells, tropomyosins are implicated in the formation and stabilization of cytoskeletal actin filaments to ensure normal cellular processes (1,2). Mutations of tropomysin-1 have been reported as a cause of dilated cardiac myopathies (4). Tropomyosin-1 also functions as a tumor suppressor, and many malignant tumors demonstrate downregulation of tropomyosin-1 expression (5-8). Tropomyosin-1 is phosphorylated at Ser283 through the Erk/DAPK pathway, which promotes stress fiber formation in response to oxidative stress (9-10).

1. Perry, S.V. (2001) J Muscle Res Cell Motil 22, 5-49.
2. Lin, J.J. et al. (2008) Adv Exp Med Biol 644, 201-22.
3. Brown, J.H. et al. (2001) Proc Natl Acad Sci USA 98, 8496-501.
4. Rajan, S. et al. (2007) Circ Res 101, 205-14.
5. Bharadwaj, S. et al. (2004) J Biol Chem 279, 14039-48.
6. Mahadev, K. et al. (2002) Exp Cell Res 279, 40-51.
7. Varga, A.E. et al. (2005) Oncogene 24, 5043-52.
8. Raval, G.N. et al. (2003) Oncogene 22, 6194-203.
9. Houle, F. et al. (2003) Mol Biol Cell 14, 1418-32.
10. Houle, F. et al. (2007) J Cell Sci 120, 3666-77.

注意事项：

Storage: Supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl,
100 µg/ml BSA, 50% glycerol and less than 0.02% sodium
azide. Store at -20°C. Do not aliquot the antibody.